Strategy for Stabilization of CutA1 Proteins Due to Ion–Ion Interactions at Temperatures of over 100 °C

In order to elucidate the contribution of charged residues to protein stabilization at temperatures of over 100 °C, we constructed many mutants of the CutA1 protein (EcCutA1) from Escherichia coli. The goal was to see if one can achieve the same stability as for a CutA1 from hyperthermophile Pyrococcus horikoshii that has the denaturation temperature near 150 °C. The hydrophobic mutant of EcCutA1 (Ec0VV) with denaturation temperature (Td) of...