Enhanced activity and stability of papain by covalent immobilization on porous magnetic nanoparticles

Papain enzyme was successfully immobilized by covalent bonding onto biocompatible Fe3O4/SF nanoparticles, which were prepared with the soft template of silk fibroin (SF). The optimized immobilization condition is pH6.0, hydrolysis time of 60min, and an enzyme/support ratio of 10.0mg/g. Compared with free papain, the immobilized papain exhibits a high effective activity, broader working pH and temperature. This immobilized papain can be separated...