The hERG PAS Domain Facilitates Gating Charge Deactivation at Physiological Temperature

The hERG PAS domain serves to suppress current, and disruption of the PAS domain enhances current in a manner that may have therapeutic potential in treating cardiac arrhythmias. We have developed two anti-PAS antibodies that interfere with PAS action and enhance current amplitude. To understand the underlying mechanism of the increased current amplitude, we tested the effect of the anti-PAS antibodies on movements of the voltage sensor domain...