Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Abstract
Class 2 CRISPR-Cas systems endow microbes with diverse mechanisms for adaptive immunity. Here, we analyzed prokaryotic genome and metagenome sequences to identify an uncharacterized family of RNA-guided, RNA-targeting CRISPR systems that we classify as type VI-D. Biochemical characterization and protein engineering of seven distinct orthologs generated a ribonuclease effector derived from Ruminococcus flavefaciens XPD3002 (CasRx) with robust...
Paper Details
Title
Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Published Date
Apr 1, 2018
Journal
Volume
173
Issue
3
Pages
665 - 676.e14
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