Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Nature64.80
Volume: 556, Issue: 7699, Pages: 57 - 63
Published: Apr 5, 2018
Abstract
A key limitation of the use of the CRISPR-Cas9 system for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the most commonly used Cas9 from Streptococcus pyogenes (SpCas9), the required PAM sequence is NGG. No natural or engineered Cas9 variants that have been shown to function efficiently in mammalian cells offer a PAM less restrictive than NGG. Here we use...
Paper Details
Title
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
Published Date
Apr 5, 2018
Journal
Volume
556
Issue
7699
Pages
57 - 63
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