CRISPR RNAs trigger innate immune responses in human cells.
Abstract
Here, we report that CRISPR guide RNAs (gRNAs) with a 5′-triphosphate group (5′-ppp gRNAs) produced via in vitro transcription trigger RNA-sensing innate immune responses in human and murine cells, leading to cytotoxicity. 5′-ppp gRNAs in the cytosol are recognized by DDX58, which in turn activates type I interferon responses, causing up to ∼80% cell death. We show that the triphosphate group can be removed by a phosphatase in vitro and that the...
Paper Details
Title
CRISPR RNAs trigger innate immune responses in human cells.
Published Date
Feb 22, 2018
Journal
Volume
28
Issue
3
Pages
367 - 373
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