Double-stranded DNA break polarity skews repair pathway choice during intrachromosomal and interchromosomal recombination

Significance DNA lesions initiated by activation-induced cytidine deaminase (AID) during class switch recombination (CSR) are diverse, and knowledge of the relationship between specific DNA lesions and DNA repair outcomes remains incomplete. Using the Cas9 endonuclease, we show that Cas9 models AID-mediated lesions leading to CSR and chromosomal translocations, and that double-stranded DNA breaks (DSBs) with 5′ overhangs skew intrachromosomal...