Surface Functionalization of Ti6Al4V via Self-assembled Monolayers for Improved Protein Adsorption and Fibroblast Adhesion

Published on Mar 20, 2018in Langmuir3.683
· DOI :10.1021/acs.langmuir.7b03152
Abshar Hasan9
Estimated H-index: 9
(IITG: Indian Institute of Technology Guwahati),
Varun Saxena7
Estimated H-index: 7
(IITG: Indian Institute of Technology Guwahati),
Lalit M. Pandey13
Estimated H-index: 13
(IITG: Indian Institute of Technology Guwahati)
Although metallic biomaterials find numerous biomedical applications, their inherent low bioactivity and poor osteointegration had been a great challenge for decades. Surface modification via silanization can serve as an attractive method for improving the aforementioned properties of such substrates. However, its effect on protein adsorption/conformation and subsequent cell adhesion and spreading has rarely been investigated. This work reports the in-depth study of the effect of Ti6Al4V surface functionalization on protein adsorption and cell behavior. We prepared self-assembled monolayers (SAMs) of five different surfaces (amine, octyl, mixed [1:1 ratio of amine:octyl], hybrid, and COOH). Synthesized surfaces were characterized by Fourier transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, contact angle goniometry, profilometry, and field emission scanning electron microscopy (FESEM). Quantification of adsorbed mass of bovine serum albumin (BSA) and fibronectin (FN) was determined on...
  • References (0)
  • Citations (19)
📖 Papers frequently viewed together
26 Citations
27 Citations
30 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
Cited By19
#1R.R. Behera (KIIT: KIIT University)
#2Apurba Das (IITG: Indian Institute of Technology Guwahati)H-Index: 2
Last. M.R. Sankar (IITs: Indian Institutes of Technology)
view all 6 authors...
Abstract The present investigation focuses on the deposition of biphasic calcium phosphate (BCP) and titania (TiO2) composite films on Ti-6Al-4V substrates using radio frequency (RF) magnetron sputtering. Three different compositions such as 100% BCP, 25% TiO2-75% BCP and 50% TiO2-50% BCP films were fabricated, and the physical, mechanical and biological behaviors of the films were analyzed. Post deposition, the films were annealed at 700 °C for 2 h to induce the crystallinity and to study its e...
#1Swati Sharma (IITG: Indian Institute of Technology Guwahati)H-Index: 5
#2Lalit M. Pandey (IITG: Indian Institute of Technology Guwahati)H-Index: 13
Abstract This study aims to unveil the effect of biosurfactant as stimulant in crude oil bioremediation. Isolated oil-degrading strain, B.subtilis RSL 2 was optimized for the maximum oil degradation and biosurfactant production using Response surface methodology. The produced biosurfactant was characterized and investigated for its effect on microbial oil degradation in two modes (a) sequential and (b) simultaneous. The strain produced 3.5 g/L of biosurfactant at pH 4.0, 25°C, using 1 g/L crude ...
#1Yan Gao (Southern Medical University)H-Index: 7
#2Ying Liu (Southern Medical University)H-Index: 1
Last. Lei Zhou (Southern Medical University)H-Index: 10
view all 9 authors...
Understanding of the complicated interactions between biomaterials and cells can accelerate biomedical developments. Recently, many in vitro studies have emphasized the biological features of nanowires (NWs) on the cell’s early response. A type of unique clustered anatase/rutile NWs (ARNWs) was generated using a simple hydrothermal reaction on titanium in the present study. The aim of this study is to determine whether the ARNWs is beneficial for the enhancement of biological capacity. Clustered...
#1Abshar Hasan (IITG: Indian Institute of Technology Guwahati)H-Index: 9
#2Lalit M. Pandey (IITG: Indian Institute of Technology Guwahati)H-Index: 13
Abstract Silanization is a widely explored chemical technique to modify surfaces for tailoring physio-chemical properties like surface chemistry and wettability but its impact on bio-interfaces interaction i.e. protein adsorption and cellular responses is not well understood. This work focusses on modulating surface properties of Ti6Al4V to study their behavior towards collagen-I (Col-I) adsorption and subsequently on osteoblast adhesion and integrin expression. The moderate hydrophobic Ti6Al4V ...
#1Andrea Toffoli (University of Parma)H-Index: 3
#2Ludovica Parisi (University of Parma)H-Index: 4
Last. Guido M. Macaluso (University of Parma)H-Index: 16
view all 6 authors...
Abstract Objectives To investigate how a thermal treatment to increase titanium wettability influences proteins adsorption from blood serum and osteoblasts responses. Methods Titanium discs with machined or micro-rough profiles were thermally treated to obtain hydrophilic surfaces. The adsorption kinetics of two representative serum proteins were determined by Bradford assay, while the stable protein adsorption pattern from blood serum was investigated by SDS-PAGE and Western Blot analysis. Subs...
3 CitationsSource
#1Aquib Jawed (IITG: Indian Institute of Technology Guwahati)H-Index: 1
#2Varun Saxena (IITG: Indian Institute of Technology Guwahati)H-Index: 7
Last. Lalit M. Pandey (IITG: Indian Institute of Technology Guwahati)H-Index: 13
view all 3 authors...
Abstract Water is polluted by various harmful toxic chemical substances, precisely heavy metals, dyes, and pathogens, which have a disastrous effect on the ecological balance of human as well as animal life. Therefore, there is an urgent need for new technologies to remove these noxious pollutants from water/wastewater. Adsorption is one of the predominant method among all the reported techniques for the removal of heavy metals. Nano technological applications in this direction, i.e. development...
3 CitationsSource
#1Kai Li (Xi'an Jiaotong University)H-Index: 1
#2Yang Xue (Xi'an Jiaotong University)H-Index: 1
Last. Yong Han (Xi'an Jiaotong University)H-Index: 32
view all 6 authors...
Extracellular Ca and Si can enhance response of osteogenesis related cells and new bone formation. Calcium silicate-base ceramics are bioactive, biodegradable and can rapidly release large amount of Ca and Si. However, the concurrently high pH value in microenvironment induce cell injury, resulting in poor osseointegration and implant loosening. In this study, NaCa2HSi3O9 nanorods were initially fabricated on Ti by micro arc oxidation and hydrothermal treatment, and then covalently immobilized w...
#1Kai LiH-Index: 1
#2Yang XueH-Index: 1
Last. Yong HanH-Index: 32
view all 5 authors...
Abstract An ideal intraosseous transcutaneous implant should form a tight seal with soft tissue, besides a requirement of osseointegration at the bone-fixed position. Si substituted hydroxyapatite (Si-HA) nanorods releasing Si ion and simulating nanotopography of natural tissue were designed on Ti to enhance fibroblast response in vitro and biosealing with soft tissue in vivo. Si-HA nanorods were fabricated by alkali-heat treatment followed with hydrothermal treatment. The hydrothermal formation...
#1Youngsik Choi (Hanyang University)H-Index: 4
#2Sicheon Seong (Hanyang University)H-Index: 2
Last. Jaegeun Noh (Hanyang University)H-Index: 24
view all 10 authors...
Abstract The surface structure, binding condition, and reductive desorption behavior of self-assembled monolayers (SAMs) of dodecyl thiocyanate (DDTC, C12–SCN) on Au(111) formed via solution and ambient-pressure vapor depositions at 50 °C for 24 h were examined by scanning tunneling microscopy (STM), X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry (CV). STM imaging clearly revealed that adsorption of DDTC molecules on Au(111) in a 1 mM ethanol solution led to the formation of shor...
#1Satish JaiswalH-Index: 2
#2Anshu DubeyH-Index: 1
Last. Debrupa LahiriH-Index: 31
view all 5 authors...
The interaction of proteins with implantable metallic surfaces has a great influence on the bioactivity and biodegradation of orthopaedic implants. Initial osseointegration is known to be critical for the long term success of orthopaedic implants. The surface properties of the implant and electrochemical milieu of the surrounding solution such as electrostatic, hydrophobic, and hydrogen bonding interactions significantly modulate protein adsorption by implants. Magnesium (Mg) is considered to im...