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Surface Functionalization of Ti6Al4V via Self-assembled Monolayers for Improved Protein Adsorption and Fibroblast Adhesion

Published on Mar 20, 2018in Langmuir3.683
· DOI :10.1021/acs.langmuir.7b03152
Abshar Hasan9
Estimated H-index: 9
(IITG: Indian Institute of Technology Guwahati),
Varun Saxena7
Estimated H-index: 7
(IITG: Indian Institute of Technology Guwahati),
Lalit M. Pandey13
Estimated H-index: 13
(IITG: Indian Institute of Technology Guwahati)
Abstract
Although metallic biomaterials find numerous biomedical applications, their inherent low bioactivity and poor osteointegration had been a great challenge for decades. Surface modification via silanization can serve as an attractive method for improving the aforementioned properties of such substrates. However, its effect on protein adsorption/conformation and subsequent cell adhesion and spreading has rarely been investigated. This work reports the in-depth study of the effect of Ti6Al4V surface functionalization on protein adsorption and cell behavior. We prepared self-assembled monolayers (SAMs) of five different surfaces (amine, octyl, mixed [1:1 ratio of amine:octyl], hybrid, and COOH). Synthesized surfaces were characterized by Fourier transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, contact angle goniometry, profilometry, and field emission scanning electron microscopy (FESEM). Quantification of adsorbed mass of bovine serum albumin (BSA) and fibronectin (FN) was determined on...
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#2Apurba Das (IITG: Indian Institute of Technology Guwahati)H-Index: 2
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Abstract The present investigation focuses on the deposition of biphasic calcium phosphate (BCP) and titania (TiO2) composite films on Ti-6Al-4V substrates using radio frequency (RF) magnetron sputtering. Three different compositions such as 100% BCP, 25% TiO2-75% BCP and 50% TiO2-50% BCP films were fabricated, and the physical, mechanical and biological behaviors of the films were analyzed. Post deposition, the films were annealed at 700 °C for 2 h to induce the crystallinity and to study its e...
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#1Swati Sharma (IITG: Indian Institute of Technology Guwahati)H-Index: 5
#2Lalit M. Pandey (IITG: Indian Institute of Technology Guwahati)H-Index: 13
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#1Yan Gao (Southern Medical University)H-Index: 7
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Understanding of the complicated interactions between biomaterials and cells can accelerate biomedical developments. Recently, many in vitro studies have emphasized the biological features of nanowires (NWs) on the cell’s early response. A type of unique clustered anatase/rutile NWs (ARNWs) was generated using a simple hydrothermal reaction on titanium in the present study. The aim of this study is to determine whether the ARNWs is beneficial for the enhancement of biological capacity. Clustered...
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#1Abshar Hasan (IITG: Indian Institute of Technology Guwahati)H-Index: 9
#2Lalit M. Pandey (IITG: Indian Institute of Technology Guwahati)H-Index: 13
Abstract Silanization is a widely explored chemical technique to modify surfaces for tailoring physio-chemical properties like surface chemistry and wettability but its impact on bio-interfaces interaction i.e. protein adsorption and cellular responses is not well understood. This work focusses on modulating surface properties of Ti6Al4V to study their behavior towards collagen-I (Col-I) adsorption and subsequently on osteoblast adhesion and integrin expression. The moderate hydrophobic Ti6Al4V ...
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Extracellular Ca and Si can enhance response of osteogenesis related cells and new bone formation. Calcium silicate-base ceramics are bioactive, biodegradable and can rapidly release large amount of Ca and Si. However, the concurrently high pH value in microenvironment induce cell injury, resulting in poor osseointegration and implant loosening. In this study, NaCa2HSi3O9 nanorods were initially fabricated on Ti by micro arc oxidation and hydrothermal treatment, and then covalently immobilized w...
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