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Remote wireless control of an enzymatic biofuel cell implanted in a rabbit for 2 months

Published on Apr 1, 2018in Electrochimica Acta5.383
· DOI :10.1016/j.electacta.2018.02.156
Sarra El Ichi-Ribault1
Estimated H-index: 1
(UGA: University of Grenoble),
Jean Pierre Alcaraz1
Estimated H-index: 1
(UGA: University of Grenoble)
+ 6 AuthorsDonald K. Martin2
Estimated H-index: 2
(UGA: University of Grenoble)
Abstract
Abstract A bioelectronic device comprising an enzymatic biofuel cell (GBFC) connected to a wireless tele-transmission system was implanted in a rabbit and its function was monitored and controlled in vivo for a period of 2 months. After the 18th day of implantation, the tele-transmission system was used to wirelessly charge and discharge the operational GBFC in vivo through a 100 kΩ load for 30 min each day. For a further 16 days of operation, the GBFC delivered 16 μW mL−1 continuously during each 30 min discharge each day for 16 days. At the end of the 2 month period of implantation the power output had diminished, which was most likely due to an inflammatory process. Our results also indicate that the duration of operational activity is increased by optimizing the interface between the GBFC and the body to minimize inflammatory processes and biofouling. These data provide a significant advance in the achievable output from a GBFC that is implanted in a mammal, and importantly provide a basis upon which to further develop stable implantable biofuel cells. Improving the in vivo performance of an implanted GBFC includes the development of biocompatible diffusing polymers to act as buffering diffusion barriers.
  • References (28)
  • Citations (12)
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References28
Newest
#1Sarra El Ichi-Ribault (French Institute of Health and Medical Research)H-Index: 2
#2Abdelkader Zebda (French Institute of Health and Medical Research)H-Index: 12
Last. Donald K. Martin (French Institute of Health and Medical Research)H-Index: 20
view all 8 authors...
Abstract A three-dimensional biocathode structure was fabricated by compacting chitosan, multiwall carbon nanotubes (MWCNTs) and laccase from Trametes versicolor in a mechanical press. The effect of functionalizing the MWCNTs with amine groups on the morphology, the electrocatalytic activity and the stability of the biocathode were evaluated for the direct and mediatorless oxygen reduction. The chemical groups bound to the MWCNTs surface influenced the interconnection of the chitosan nanofibers ...
4 CitationsSource
#1Sergey Shleev (Malmö University)H-Index: 38
The road to safe and effective implantable electrical power devices has been long—and the goal has not been reached yet, although a certain amount of scientific and technological progress has been made. This brief review is focused on highlighting the stages of development of implanted fuel cells capable of providing electrical power for running implanted “personal electronics”. The paper starts with early efforts to implant glucose-burning fuel cells in dogs, and ends with realistic attempts at...
14 CitationsSource
#1Jean-Pierre Alcaraz (French Institute of Health and Medical Research)H-Index: 3
#2Sarra El Ichi-Ribault (French Institute of Health and Medical Research)H-Index: 2
Last. Donald K. Martin (French Institute of Health and Medical Research)H-Index: 20
view all 7 authors...
Une biopile enzymatique implantable est un dispositif produisant du courant uniquement a partir du glucose et de l’oxygene naturellement et constamment presents dans l’organisme. Les enzymes presentes aux electrodes oxydent le glucose et reduisent l’oxygene, ce qui genere un flux d’electrons dans un circuit electrique associe. Cette microcentrale electrique, qui peut fournir une tension de presque un volt, pourra alimenter sur une longue periode la future generation de robots implantables. Tout ...
3 CitationsSource
#1Kan Shoji (Osaka University)H-Index: 4
#2Yoshitake Akiyama (Osaka University)H-Index: 13
Last. Keisuke Morishima (Osaka University)H-Index: 19
view all 6 authors...
Abstract This study investigated an enzymatic biofuel cell (BFC) which can be backpacked by cockroaches. The BFC generates electric power from trehalose in insect hemolymph by the trehalase and glucose dehydrogenase (GDH) reaction systems which dehydrogenate β-glucose obtained by hydrolyzing trehalose. First, an insect-mountable BFC (imBFC) was designed and fabricated with a 3D printer. The electrochemical reaction of anode-modified poly- l -lysine, vitamin K3, diaphorase, nicotinamide adenine d...
18 CitationsSource
#2Magnus FalkH-Index: 13
Last. Dónal LeechH-Index: 36
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Coimmobilization of pyranose dehydrogenase as an enzyme catalyst, osmium redox polymers [Os(4,4′-dimethoxy-2,2′-bipyridine)2(poly(vinylimidazole))10Cl]+ or [Os(4,4′-dimethyl-2,2′-bipyridine)2(poly(vinylimidazole))10Cl]+ as mediators, and carbon nanotube conductive scaffolds in films on graphite electrodes provides enzyme electrodes for glucose oxidation. The recombinant enzyme and a deglycosylated form, both expressed in Pichia pastoris, are investigated and compared as biocatalysts for glucose ...
38 CitationsSource
#1Anne Riemann (Wittenberg University)H-Index: 9
#2Hanna Wußling (Wittenberg University)H-Index: 1
Last. Oliver Thews (Wittenberg University)H-Index: 30
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Inflammation, ischemia or the microenvironment of solid tumors is often accompanied by a reduction of extracellular pH (acidosis) that stresses the cells and acts on cellular signaling and transcription. The effect of acidosis on the expression of various inflammatory markers, on functional parameters (migration, phagocytic activity) and on signaling pathways involved was studied in monocytic cells and macrophages. In monocytic cell lines acidosis led to a reduction in expression of most of the ...
17 CitationsSource
#1S. El Ichi (French Institute of Health and Medical Research)H-Index: 1
#2Abdelkader Zebda (French Institute of Health and Medical Research)H-Index: 12
Last. Donald K. Martin (French Institute of Health and Medical Research)H-Index: 20
view all 11 authors...
A 3D nanofibrous network of compressed chitosan in the presence of genipin as the cross-linker, carbon nanotubes and laccase constitutes a new design to enhance the stability and the biocompatibility of biocathodes. The in vitro delivered current was around −0.3 mA mL−1 for 20 days under continuous discharge. A thin film made of chitosan cross-linked with genipin was synthesized and optimized for oxygen and glucose diffusion. This film was used as a biocompatible barrier on the surface of biocat...
28 CitationsSource
#1Sarra El Ichi (CNRS: Centre national de la recherche scientifique)H-Index: 1
#2Abdelkader Zebda (CNRS: Centre national de la recherche scientifique)H-Index: 12
Last. Donald K. Martin (CNRS: Centre national de la recherche scientifique)H-Index: 20
view all 7 authors...
The present work demonstrates the in vivo biocompatibility and operation of a biofuel cell enclosed by a chitosan membrane. The measured power output in the range of 6.2 to 20.7 µW cm−2 (applied current from 10 to 70 µA) indicated that glucose and oxygen can reach the enzymes in the 3D matrix without any need for a buffer solution in the implant or a hydration step. This is very important to simplify the preparation and sterilization steps, to avoid contamination and to favour the close communic...
3 CitationsSource
#1Sarra El Ichi (UGA: University of Grenoble)H-Index: 1
#2Abdelkader Zebda (UGA: University of Grenoble)H-Index: 2
Last. Donald K. Martin (UGA: University of Grenoble)H-Index: 2
view all 8 authors...
We demonstrate a novel combined chitosan–carbon-nanotube–enzyme biocathode with a greatly enhanced and stable long-term current density of −0.19 mA mL−1. The fibrous microstructure of the electrode improves the performance of the biocathode by creating a protective microenvironment, preventing the loss of the electrocatalytic activity of the enzyme, and providing good oxygen diffusion.
22 CitationsSource
#1Magnus Falk (Malmö University)H-Index: 13
#2Miguel AlcaldeH-Index: 39
Last. Sergey Shleev (Malmö University)H-Index: 38
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Here for the first time, we detail self-contained (wireless and self-powered) biodevices with wireless signal transmission. Specifically, we demonstrate the operation of self-sustained carbohydrate and oxygen sensitive biodevices, consisting of a wireless electronic unit, radio transmitter and separate sensing bioelectrodes, supplied with electrical energy from a combined multi-enzyme fuel cell generating sufficient current at required voltage to power the electronics. A carbohydrate/oxygen enzy...
82 CitationsSource
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Abstract Enzymatic biofuel cells (EBFCs) have attracted increasing attention due to their potential to harvest energy from a wide range of fuels under mild conditions. Fabrication of effective bioelectrodes is essential for the practical application of EBFCs. Graphene possesses unique physiochemical properties making it an attractive material for the construction of EBFCs. Despite these promising properties, graphene has not been used for EBFCs as frequently as carbon nanotubes, another nanoscal...
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Abstract Genipin is used to simultaneously crosslink chitosan, amine-containing osmium redox complex and glucose oxidase on graphite disks to produce enzyme-based electrodes for glucose oxidation. The enzyme electrodes produce glucose oxidation current densities of 730 µA cm−2 in 50 mM phosphate buffered saline (150 mM NaCl, pH 7.4, 37 °C) containing 100 mM glucose at an applied potential of 0.45 V (vs. Ag/AgCl), higher than the 440 µA cm−2 for conventional poly(ethylene glycol) diglycidyl ether...
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Abstract In this study, a high-power, non-enzymatic glucose biofuel cell (GBFC) based on a nano/micro hybrid-structured Au anode is proposed. A uniformly distributed micro-hemispheric array of polycarbonate is fabricated for this novel electrode by hot embossing using a Ni mold. The nano/micro hybrid-structured Au anode is then fabricated by depositing an Au nanoparticle monolayer on the micro-hemispheric array using 1,6-hexanedithiol as a two-sided anchor. The cathode is composed of a graphene ...
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