Conditional Manipulation of Gene Function in Human Cells with Optimized Inducible shRNA

The difficulties involved in conditionally perturbing complex gene expression networks represent major challenges toward defining the mechanisms controlling human development, physiology, and disease. We developed an OPTimized inducible KnockDown (OPTiKD) platform that addresses the limitations of previous approaches by allowing streamlined, tightly‐controlled, and potent loss‐of‐function experiments for both single and multiple genes. The...