Myosin light chain kinase (MYLK) coding polymorphisms modulate human lung endothelial cell barrier responses via altered tyrosine phosphorylation, spatial localization, and lamellipodial protrusions

Sphingosine 1‐phosphate (S1P) is a potent bioactive endogenous lipid that signals a rearrangement of the actin cytoskeleton via the regulation of non‐muscle myosin light chain kinase isoform (nmMLCK). S1P induces critical nmMLCK Y 464 and Y 471 phosphorylation resulting in translocation of nmMLCK to the periphery where spatially‐directed increases in myosin light chain (MLC) phosphorylation and tension result in lamellipodia protrusion,...