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Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

Published on Mar 1, 2018
· DOI :10.1007/s13337-018-0430-z
David De la Torre2
Estimated H-index: 2
(USP: University of São Paulo),
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo)
+ 2 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
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Abstract
Avian adenovirus has been reported in many countries and is an infectious agent related with inclusion body hepatitis, hepatitis-hydropericardium syndrome (HHS), and respiratory and enteric conditions in chickens worldwide. The objective of this study was to detect and establish the molecular sequences of the hexon gene from the avian adenovirus strains of group I (FAdV-I) isolated from birds with hepatitis-hydropericardium syndrome (HHS), malabsorption syndrome and runting-stunting syndrome, to characterize the serotype of virus affecting commercial flocks in Brazil. Molecular characterization was performed by polymerase chain reaction (PCR), using specific primers to amplify the Loop 1 (L1) variable region of the hexon gene in the FAdV-I genome and subsequent sequencing of the PCR product for each positive sample. The results have revealed the presence of the FAdV-8a, FAdV-8b, and FAdV-11 serotypes circulating in Brazilian chicken flocks. Phylogenetic analysis grouped these sequences into three (3) distinct groups, 14 samples were aligned with the FAdV-11 group, three (3) samples in the FAdV-8b group and one (1) sample in the FAdV-8a group. The serotypes FAdV-8a, FAdV-8b, and FAdV-11 are circulating in Brazilian chicken flocks. Therefore, these results are very important for improvement biosecurity measurements and vaccine production.
  • References (30)
  • Citations (2)
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References30
Newest
Published on Jul 1, 2016in Pesquisa Veterinaria Brasileira 0.30
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo),
Silvana Hipatia Santander Parra5
Estimated H-index: 5
(USP: University of São Paulo)
+ 4 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Enteric disease is a multifactorial problem in chickens, which causes gastrointestinal alterations, elevated feed conversions and impairment. In the last years, several enteric viruses were implicated in enteric disease; case reports have shown their presence alone or in concomitant infections during outbreaks and have suggested that they might be determining factors in the aetiology of enteric disease. This study shows high detection rates of enteric viruses in the pancreas and spleen in sample...
Published on Apr 28, 2016in Genome Announcements
Lintao Li2
Estimated H-index: 2
(HAU: Huazhong Agricultural University),
Ling Luo4
Estimated H-index: 4
+ 9 AuthorsHuabin Shao5
Estimated H-index: 5
ABSTRACT We report here the complete genome sequence of virulent fowl adenovirus serotype 4 strain HB1510, isolated from a diseased chicken with hydropericardium-hepatitis syndrome in Hubei, China, in 2015. The viral genome is 43,721 bp long, and sequence analysis showed an 11-amino-acid deletion in open reading frame 29 (ORF29).
Published on Jan 1, 2016in Archives of Virology 2.26
Jowita Samanta5
Estimated H-index: 5
Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbreaks and developing strategies for vaccine development. Several molecular epidemiology and phylogenetic studies have been performed, but the results obtained are still limited, becau...
Published on Dec 1, 2015in Acta Veterinaria Hungarica 1.06
Vasudevan Gowthaman4
Estimated H-index: 4
,
Sambu Dhayal Singh1
Estimated H-index: 1
+ 7 AuthorsMuthannan Andavar Ramakrishnan13
Estimated H-index: 13
Avian nephritis virus (ANV) infects poultry flocks worldwide, but no confirmed cases have been reported from India so far. In the current study, disease investigation was carried out in 21 broiler flocks at different parts of India with clinical signs of nephritis, uneven and stunted growth, diarrhoea, reduced body weight, and mortality up to 9.72%. Out of the 21 flocks screened, two were found positive for ANV in RT-PCR assay. BLAST analysis revealed that the ANV of Indian origin was closely re...
Published on Mar 4, 2015in Avian Pathology 1.96
Penelope A. Steer7
Estimated H-index: 7
(UNE: University of New England (Australia)),
Jeanine R. Sandy3
Estimated H-index: 3
(University of Melbourne)
+ 3 AuthorsAmir H. Noormohammadi24
Estimated H-index: 24
(University of Melbourne)
Fowl adenoviruses (FAdVs) cause diseases in domestic chickens, including inclusion body hepatitis (IBH), with immunosuppression believed to play a role in their pathogenesis. To gain a better understanding of the pathogenesis and chronology of disease caused by FAdVs, the gross pathology, histopathology and dissemination of virus were examined at several different time points, after inoculation of one-day-old specific pathogen-free chickens with FAdV-1, FAdV-8b or FAdV-11 via the ocular route. F...
Published on Aug 1, 2014in Pesquisa Veterinaria Brasileira 0.30
Elena Mettifogo9
Estimated H-index: 9
(USP: University of São Paulo),
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo)
+ 2 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Lotes comerciais de frangos de uma granja localizada no Estado de Sao Paulo, Brasil, apresentavam diarreia, depressao, aumento de mortalidade e baixo ganho de peso. Apos o exame post-mortem, sinais classicos da sindrome de hepatite por corpusculo de inclusao/hidropericardio (IBH/HPS) foram observados incluindo hepatomegalia com aspecto amarelado palido e liquido de coloracao amarelo palha no saco pericardio. Alem disso, as alteracoes macroscopicas foram tambem observadas nos rins, pâncreas, timo...
Published on Jan 1, 2014in Ai Magazine 1.32
Jorge Rodríguez2
Estimated H-index: 2
,
Ysabel Koga2
Estimated H-index: 2
+ 1 AuthorsRobert Tinoco1
Estimated H-index: 1
Forty seven clinical samples of Fowl adenovirus (FAdV) associated with Inclusion Body Hepatitis (IBH) from Peruvian broilers received between July 2006 and April 2013 were genotyped using sequencing of L1 Loop of Hexon gene. All 47 clinical samples presented macroscopic and histopathology lesions consistent with IBH, and amplified a specific fragment of Hexon gene by Polymerase Chain Reaction (PCR). A unique nucleotide sequence of 789 base pairs of Hexon gene (position 273 to 1061) was obtained ...
Published on Jan 1, 2014in The Scientific World Journal
Elena Mettifogo9
Estimated H-index: 9
,
Luis Fabian N Nuñez5
Estimated H-index: 5
+ 5 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Several viruses have been identified in recent years in the intestinal contents of chickens and turkeys with enteric problems, which have been observed in commercial farms worldwide, including Brazil. Molecular detection of these viruses in Brazil can transform to a big threat for poultry production due to risk for intestinal integrity. This disease is characterized by severely delayed growth, low uniformity, lethargy, watery diarrhea, delayed feed consumption, and a decreased conversion rate. C...
Published on Jan 1, 2014
D. Mittal5
Estimated H-index: 5
,
Naresh Jindal10
Estimated H-index: 10
(Lala Lajpat Rai University of Veterinary and Animal Sciences)
+ 1 AuthorsRaj Singh Khokhar1
Estimated H-index: 1
(Lala Lajpat Rai University of Veterinary and Animal Sciences)
The present study was carried out for detection and molecular characterization of fowl adenoviruses (FAdVs) associated with hydropericardium syndrome or inclusion body hepatitis in commercial broiler chickens. The FAdVs were detected in liver samples from 33 commercial broiler chicken flocks by polymerase chain reaction (PCR) using hexon gene specific primers. The restriction enzyme analysis using StyI, BsiWI, MluI, AspI, BglI and ScaI enzymes of all the 33 FAdV-positive samples revealed FAdV-4 ...
Published on Dec 1, 2013in Worlds Poultry Science Journal 1.36
L. F. N. Nuñez4
Estimated H-index: 4
(USP: University of São Paulo),
A. J. Piantino Ferreira6
Estimated H-index: 6
(USP: University of São Paulo)
Cited By2
Newest
Published on May 1, 2019in Transboundary and Emerging Diseases 3.55
Arianne Brown Jordan3
Estimated H-index: 3
(UWI: University of the West Indies),
Lemar Blake2
Estimated H-index: 2
(UWI: University of the West Indies)
+ 3 AuthorsC.A.L. Oura26
Estimated H-index: 26
(UWI: University of the West Indies)
Published on Nov 1, 2018in Poultry Science 2.03
Helena Redondo1
Estimated H-index: 1
,
Jaime Sarabia Fragoso1
Estimated H-index: 1
+ 5 AuthorsJuan Carlos Abad Moreno1
Estimated H-index: 1