Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil
Published on Mar 1, 2018
· DOI :10.1007/s13337-018-0430-z
Avian adenovirus has been reported in many countries and is an infectious agent related with inclusion body hepatitis, hepatitis-hydropericardium syndrome (HHS), and respiratory and enteric conditions in chickens worldwide. The objective of this study was to detect and establish the molecular sequences of the hexon gene from the avian adenovirus strains of group I (FAdV-I) isolated from birds with hepatitis-hydropericardium syndrome (HHS), malabsorption syndrome and runting-stunting syndrome, to characterize the serotype of virus affecting commercial flocks in Brazil. Molecular characterization was performed by polymerase chain reaction (PCR), using specific primers to amplify the Loop 1 (L1) variable region of the hexon gene in the FAdV-I genome and subsequent sequencing of the PCR product for each positive sample. The results have revealed the presence of the FAdV-8a, FAdV-8b, and FAdV-11 serotypes circulating in Brazilian chicken flocks. Phylogenetic analysis grouped these sequences into three (3) distinct groups, 14 samples were aligned with the FAdV-11 group, three (3) samples in the FAdV-8b group and one (1) sample in the FAdV-8a group. The serotypes FAdV-8a, FAdV-8b, and FAdV-11 are circulating in Brazilian chicken flocks. Therefore, these results are very important for improvement biosecurity measurements and vaccine production.