Mapping Transposon Insertions in Bacterial Genomes by Arbitrarily Primed PCR
Abstract
Transposons can be used to easily generate and label the location of mutations throughout bacterial and other genomes. Transposon insertion mutants may be screened for a phenotype as individual isolates, or by selection applied to a pool of thousands of mutants. Identifying the location of a transposon insertion is critical for connecting phenotype to the genetic lesion. In this unit, we present an easy and detailed approach for mapping...
Paper Details
Title
Mapping Transposon Insertions in Bacterial Genomes by Arbitrarily Primed PCR
Published Date
Apr 1, 2017
Volume
118
Issue
1
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