CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep

One of the ultimate goals of regenerative medicine is the generation of patient-specific organs from pluripotent stem cells (PSCs). Sheep are potential hosts for growing human organs through the technique of blastocyst complementation. We report here the creation of pancreatogenesis-disabled sheep by oocyte microinjection of CRISPR/Cas9 targeting PDX1 , a critical gene for pancreas development. We compared the efficiency of target mutations...