Distinct roles of N- and O-glycans in cellulase activity and stability

Significance Glycosylation is a ubiquitous posttranslational modification of proteins wherein carbohydrates are appended to protein side chains, with myriad functions in molecular and cell biology. The enzymes that break down polysaccharides and other recalcitrant polymers in nature are often decorated with two canonical forms of glycosylation, N- and O-linked glycans, the roles of which are only partially understood in these key enzyme families...