Simultaneous determination of Vitamin A, 25-hydroxyl vitamin D 3 α-tocopherol in small biological fluids by liquid chromatography-tandem mass spectrometry

A sensitive method for the simultaneous analysis of vitamin A (VA), 25-hydroxyl vitamin D3 (25-OH VD3) and α-tocopherol (VE) in children plasma by liquid chromatography-tandem mass spectrometry (LC–MS/MS) has been developed and validated. Sample preparation chose the solid phase extraction. 100 μL of plasma was mixed with 300 μL ethanol contained 4 μL isotope-labelled analytes. After a series operation, the supernatant was applied to the solid...