Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis
Volume: 105, Pages: 1 - 22
Published: Apr 1, 2018
Abstract
Preparation of a protein sample for liquid-state nuclear magnetic resonance (NMR) spectroscopy analysis requires optimization of many parameters. This review describes labeling strategies for obtaining assignments of protein resonances. Particular emphasis is placed on the advantages of cell-free protein production, which enables exclusive labeling of the protein of interest, thereby simplifying downstream processing steps and increasing the...
Paper Details
Title
Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis
Published Date
Apr 1, 2018
Volume
105
Pages
1 - 22
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