Effects of voltage strength during electroporation on the development and quality of in vitro‐produced porcine embryos

Contents This study was conducted to determine suitable conditions for an experimental method in which the CRISPR /Cas9 system is introduced into in vitro‐produced porcine zygotes by electroporation. In the first experiment, when putative zygotes derived from in vitro fertilization ( IVF ) were electroporated by either unipolar or bipolar pulses, keeping the voltage, pulse duration and pulse number fixed at 30 V/mm, 1 msec and five repeats,...