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Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

Published on Oct 2, 2017in Mycology
· DOI :10.1080/21501203.2017.1385546
Wen-ming Cheng1
Estimated H-index: 1
(Anhui Medical University),
Xun Zhang1
Estimated H-index: 1
(Anhui Medical University)
+ 4 AuthorsChun-ru Li1
Estimated H-index: 1
(AAU: Anhui Agricultural University)
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Abstract
ABSTRACTNucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine Ophiocordyceps species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of Cordyceps were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of Ophiocordyceps sinensis (NFOS). Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, ad...
  • References (19)
  • Citations (5)
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References19
Newest
Published on Mar 1, 2017in Acta Pharmaceutica Sinica B
Zhengming Qian6
Estimated H-index: 6
(UM: University of Macau),
Shao-Ping Li31
Estimated H-index: 31
(UM: University of Macau)
Abstract Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) which were controlled by column-switching valves. The sample was separated by the size exclusion p...
Published on Jan 1, 2014in Journal of Pharmaceutical and Biomedical Analysis2.98
Jing Zhao28
Estimated H-index: 28
(UM: University of Macau),
Jing Xie9
Estimated H-index: 9
(UM: University of Macau)
+ 1 AuthorsS.P. Li31
Estimated H-index: 31
(UM: University of Macau)
Abstract Cordyceps sinensis , also called DongChongXiaCao (winter worm summer grass) in Chinese, is a well-known and valued traditional Chinese medicine. In 2006, we wrote a review for discussing the markers and analytical methods in quality control of Cordyceps (J. Pharm. Biomed. Anal. 41 (2006) 1571–1584). Since then this review has been cited by others for more than 60 times, which suggested that scientists have great interest in this special herbal material. Actually, the number of publicati...
Published on Jul 1, 2013in Journal of Functional Foods3.20
M.G. Shashidhar1
Estimated H-index: 1
(AcSIR: Academy of Scientific and Innovative Research),
P. Giridhar9
Estimated H-index: 9
(CSIR-CFTRI: Central Food Technological Research Institute)
+ 1 AuthorsBalaraman Manohar15
Estimated H-index: 15
(AcSIR: Academy of Scientific and Innovative Research)
Cordyceps sinensis (CS) is a well-known entamophagus fungus, naturally distributed in the Tibetan Plateau of Asia and Himalayas. Recently this synonym is transferred to Ophiocordyceps by both scientific and non-scientific communities. It is widely used as a tonic and medicinal food in traditional Chinese medicine (TCM), as it possess wonderful health benefits. To support its functional attributes, various investigations have been carried out to find out its adaptogenic, aphrodisiac, anti-oxidant...
Hui-Chen Lo10
Estimated H-index: 10
(FJU: Fu Jen Catholic University),
Chienyan Hsieh1
Estimated H-index: 1
(NKNU: National Kaohsiung Normal University)
+ 1 AuthorsTai-Hao Hsu12
Estimated H-index: 12
ABSTRACT The caterpillar fungus Ophiocordyceps sinensis (syn. † Cordyceps sinensis ), which was originally used in traditional Tibetan and Chinese medicine, is called either “yartsa gunbu” or “DongChongXiaCao (冬蟲夏草 Dōng Chong Xia Cǎo)” (“winter worm-summer grass”), respectively. The extremely high price of DongChongXiaCao, approximately USD $20,000 to 40,000 per kg, has led to it being regarded as “soft gold” in China. The multi-fungi hypothesis has been proposed for DongChongXiaCao; however, Hi...
Published on Dec 1, 2009in Journal of Separation Science2.52
Shuang Wang1
Estimated H-index: 1
(UM: University of Macau),
Feng-Qing Yang24
Estimated H-index: 24
(Chongqing University)
+ 3 AuthorsShao-Ping Li31
Estimated H-index: 31
(UM: University of Macau)
A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed. A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 9...
Published on Oct 1, 2009in Journal of Pharmaceutical and Biomedical Analysis2.98
Feng-Qing Yang24
Estimated H-index: 24
(UM: University of Macau),
Liya Ge21
Estimated H-index: 21
(NTU: Nanyang Technological University)
+ 2 AuthorsShao-Ping Li31
Estimated H-index: 31
(UM: University of Macau)
Abstract In present study, a capillary electrophoresis–mass spectrometry (CE–MS) method was developed for the simultaneous analysis of 12 nucleosides and nucleobases including cytosine, adenine, guanine, cytidine, cordycepin, adenosine, hypoxanthine, guanosine, inosine, 2′-deoxyuridine, uridine and thymidine in natural and cultured Cordyceps using 5-chlorocytosine arabinoside as an internal standard (IS). The CE separation conditions and MS parameters were optimized systematically for achieving ...
Published on Mar 1, 2009in Journal of Pharmacy and Pharmacology2.39
Xuanwei Zhou15
Estimated H-index: 15
(SJTU: Shanghai Jiao Tong University),
Zhenghua Gong1
Estimated H-index: 1
(SJTU: Shanghai Jiao Tong University)
+ 2 AuthorsKexuan Tang29
Estimated H-index: 29
(Fudan University)
Objectives Parasitic Cordyceps fungi, such as Cordyceps sinensis, is a parasitic complex of fungus and caterpillar, which has been used for medicinal purposes for centuries particularly in China, Japan and other Asian countries. This article gives a general idea of the latest developments in C. sinensis research, with regard to the active chemical components, the pharmacological effects and the research and development of products in recent years. Key findings The common names for preparations i...
Published on Jun 1, 2008in Biomedical Chromatography1.75
Rie Ikeda10
Estimated H-index: 10
(Nagasaki University),
Miho Nishimura1
Estimated H-index: 1
(Nagasaki University)
+ 2 AuthorsKenichiro Nakashima31
Estimated H-index: 31
(Nagasaki University)
A simple HPLC-UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2′-deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C18 column by isocratic elution with acetonitrile–water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2′-deoxyadenosine, guanosine and uridine in Cordyceps were 0.28–14.15, 0.006–6.36, 0.01–0.14, 0.68–14.79 and 0.19–20....
Published on Sep 15, 2007in Talanta4.92
Feng-Qing Yang24
Estimated H-index: 24
(UM: University of Macau),
J. Guan3
Estimated H-index: 3
(UM: University of Macau),
Shao-Ping Li31
Estimated H-index: 31
(UM: University of Macau)
Abstract Determination of nucleosides and their metabolic compounds is important for physiological and pharmacological studies. Herein, a rapid ultra-performance liquid chromatography (UPLC) method was developed for the simultaneous determination of 14 nucleosides and nucleobases, namely adenine, adenosine, cytosine, cytidine, uracil, uridine, guanine, guanosine, hypoxanthin, inosine, thymine, thymidine, 2′-deoxyuridine and cordycepin. The separation was performed on Waters Acquity UPLC system w...
Published on Dec 1, 2006in Journal of Food Composition and Analysis2.99
C. Li1
Estimated H-index: 1
(AAU: Anhui Agricultural University),
Zengzhi Li11
Estimated H-index: 11
(AAU: Anhui Agricultural University)
+ 4 AuthorsL. Ming1
Estimated H-index: 1
(Anhui Medical University)
Abstract The compositions of natural fruiting bodies of Cordyceps sinensis (NFCS), mycelia from submerged culture (MSMC) and shake culture (MSKC) of RCEF0273, a strain of Hirsutella sinensis , anamorph of C. sinensis , were compared to evaluate the potentiality of MSMC. The contents of crude fat, crude protein, total and essential amino acids were in the following descending order: MSKC>MSMC>NFCS, respectively. Unsaturated fatty acids of MSMC accounted for 65.9% of total fatty acids, obviously l...
Cited By5
Newest
Published on Jun 1, 2019in Journal of Ethnopharmacology3.41
Jin-Xiu Liang1
Estimated H-index: 1
(CPU: China Pharmaceutical University),
Qun-Qun Zhang1
Estimated H-index: 1
(CPU: China Pharmaceutical University)
+ 5 AuthorsHua Yang15
Estimated H-index: 15
(CPU: China Pharmaceutical University)
Abstract Ethnopharmacological relevance Monascus -fermented rice product (MFRP) has been regarded as a dietary supplement and traditional medicine with circulation-promoting effects in China and other countries for centuries. Aim of the study This study was carried out to profile the chemical components in MFRP, and provide available information for elucidating the potential lipid-lowering compounds other than monacolins. Materials and methods High-performance liquid chromatography coupled with ...
Published on May 1, 2019in Journal of Chromatographic Science1.22
Caifeng Meng (Hebei Medical University), Qingjie Han (Hebei Medical University)+ 5 AuthorsChunying Wang5
Estimated H-index: 5
(Hebei Medical University)
Published on Dec 1, 2018in BMC Complementary and Alternative Medicine2.48
Mei Ge (ECNU: East China Normal University), Rui Guo (ECNU: East China Normal University)+ 1 AuthorsWen Zhang (ECNU: East China Normal University)
Background Cordyceps sinensis has been used for centuries in China as one of the most valued herbal medicine and tonic food. Paecilomyces hepiali, a fungal strain isolated from natural C. sinensis, has been used widely as a substitute of C. sinensis in medicine and health food. P. hepiali has been reported to have various pharmaceutical benefits, including triglyceride-lowing activity. However, its effects on triglyceride metabolism in adipocytes remain unknown. The purpose of the present study ...
Published on Oct 2, 2018in Journal of Liquid Chromatography & Related Technologies0.99
Robin Joshi12
Estimated H-index: 12
(CSIR: Council of Scientific and Industrial Research),
Aakriti Sharma (CSIR: Council of Scientific and Industrial Research)+ 2 AuthorsGireesh Nadda6
Estimated H-index: 6
(CSIR: Council of Scientific and Industrial Research)
AbstractA fast, reliable and reproducible UHPLC-IMS method was developed and validated for simultaneous determination of eight nucleosides (adenine, adenosine, cordycepin, guanosine, inosine, thymidine, thymine and uracil) in natural Ophiocordyceps sinensis. Present study was conducted to determine the marker nucleoside contents in low quantities using the developed method. Methanol and water in combination were used as extraction solvents due to hydrophilic nature of nucleosides. These nucleosi...
Published on Sep 1, 2018in Fitoterapia2.43
Opeyemi Joshua Olatunji3
Estimated H-index: 3
(PSU: Prince of Songkla University),
Jian Tang4
Estimated H-index: 4
(Jiangsu University)
+ 3 AuthorsZhen Ouyang5
Estimated H-index: 5
(Jiangsu University)
Abstract The genus Cordyceps has about 750 identified species which are distributed in many regions of the World, but mainly found in South Asia, Europe and North America. The species of the genus are highly priced and are widely used as food and medicine for the treatment of various ailments. This present review compiles literature information on the ethno-medicinal, phytochemistry and pharmacological properties of species in the genus Cordycep s. In addition, the review also suggest recommenda...
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