Recombinase polymerase amplification: Basics, applications and recent advances
Abstract
Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal amplification technique, operating at 37-42°C, with minimal sample preparation and capable of amplifying as low as 1-10 DNA target copies in less than 20 min. It has been used to amplify diverse targets, including RNA, miRNA, ssDNA and dsDNA from a wide variety of organisms and samples. An ever increasing number of publications detailing the use of RPA are...
Paper Details
Title
Recombinase polymerase amplification: Basics, applications and recent advances
Published Date
Jan 1, 2018
Volume
98
Pages
19 - 35
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