pH Effects on Chromatographic Retention Modes

Pages: 263 - 278
Published: Nov 25, 2015
Abstract
The pH of a mobile phase is one of the most important parameters affecting retention of ionic analytes in reversed phase high-performance liquid chromatography (RP-HPLC). RP-HPLC and hydrophilic interaction liquid chromatography (HILIC) can be used as a convenient tool for the analysis of ionizable analytes. The ionic analyte contains one or more acidic or basic functional groups in its molecular structure. Acidic compounds lose a proton as pH...
Paper Details
Title
pH Effects on Chromatographic Retention Modes
Published Date
Nov 25, 2015
Journal
Pages
263 - 278
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