Ultrasonic-based membrane aided sample preparation of urine proteomes
Abstract
A new ultrafast ultrasonic-based method for shotgun proteomics as well as label-free protein quantification in urine samples is developed. The method first separates the urine proteins using nitrocellulose-based membranes and then proteins are in-membrane digested using trypsin. The enzymatic digestion process is accelerated from overnight to four minutes using a sonoreactor ultrasonic device. Overall, the sample treatment pipeline comprising...
Paper Details
Title
Ultrasonic-based membrane aided sample preparation of urine proteomes
Published Date
Feb 1, 2018
Journal
Volume
178
Pages
864 - 869
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Notes
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