The effect of cations on reversibility and thermodynamic stability during thermal denaturation of lysozyme

Differential scanning calorimetry (DSC) has been used to investigate the role that cations of different salts (CaCl2, MgCl2, NH4Cl, NaCl and KCl) play on the thermodynamic stability and reversibility of the lysozyme’s folding-unfolding process. In this work, thermodynamic parameters such as enthalpy change ΔΗ, apparent heat capacity change (ΔCp) and the melt transition temperature (Tm) were obtained in order to explore the effects of various...