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Inward H+ pump xenorhodopsin: Mechanism and alternative optogenetic approach

Published on Sep 1, 2017in Science Advances
· DOI :10.1126/sciadv.1603187
Vitaly Shevchenko6
Estimated H-index: 6
(MIPT: Moscow Institute of Physics and Technology),
Thomas Mager6
Estimated H-index: 6
(MPG: Max Planck Society)
+ 18 AuthorsValentin I. Gordeliy23
Estimated H-index: 23
(MIPT: Moscow Institute of Physics and Technology)
Abstract
Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives of the yet noncharacterized xenorhodopsins from Nanohaloarchaea family of microbial rhodopsins. They are inward proton pumps as we demonstrate in model membrane systems, Escherichia coli cells, human embryonic kidney cells, neuroblastoma cells, and rat hippocampal neuronal cells. We also solved the structure of a xenorhodopsin from the nanohalosarchaeon Nanosalina (NsXeR) and suggest a mechanism of inward proton pumping. We demonstrate that the NsXeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency. Hence, inwardly directed proton pumps are suitable for light-induced remote control of neurons, and they are an alternative to the well-known cation-selective channelrhodopsins.
  • References (30)
  • Citations (11)
References30
Newest
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#2Vitaly Shevchenko (MIPT: Moscow Institute of Physics and Technology)H-Index: 6
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#1Oliver P. Ernst (U of T: University of Toronto)H-Index: 34
#2David T. LodowskiH-Index: 18
Last.Hideki Kandori (Nagoya Institute of Technology)H-Index: 41
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#1A. I. Kuzmich (RAS: Russian Academy of Sciences)H-Index: 3
#2A. V. Vvedenskii (RAS: Russian Academy of Sciences)H-Index: 1
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#2Ulrich Terpitz (MPG: Max Planck Society)H-Index: 7
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#1Keiichi InoueH-Index: 21
#2María del Carmen Marín (UNISI: University of Siena)H-Index: 2
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#2Wei-Lin Ou (U of T: University of Toronto)H-Index: 4
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#2Arita Silapetere (Humboldt University of Berlin)H-Index: 2
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#2Vitaly Polovinkin (CNRS: Centre national de la recherche scientifique)H-Index: 1
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