Quantification of Lignin and Its Structural Features in Plant Biomass Using ¹³C Lignin as Internal Standard for Pyrolysis-GC-SIM-MS

Understanding the mechanisms underlying plant biomass recalcitrance at the molecular level can only be achieved by accurate analyses of both the content and structural features of the molecules involved. Current quantification of lignin is, however, majorly based on unspecific gravimetric analysis after sulfuric acid hydrolysis. Hence, our research aimed at specific lignin quantification with concurrent characterization of its structural...