Enhancing recombinant interleukin‐6 production yield by fermentation optimization, two‐step denaturing, and one‐step purification

Interleukin‐6 a pleiotropic cytokine involved in a wide range of biological activities. So the large‐scale production of biologically active recombinant human interleukin‐6 is important for its structural and functional studies. Here, we report an optimized method for shake flask fermentation and a simplified high‐yield purification procedure for the recombinant interleukin‐6. This high‐yield expression method not only involves the optimization...