Match!

Genetic characterisation and analysis of infectious bronchitis virus isolated from Brazilian flocks between 2010 and 2015.

Published on Oct 17, 2017in British Poultry Science1.421
· DOI :10.1080/00071668.2017.1365116
Claudia Carranza3
Estimated H-index: 3
(USP: University of São Paulo),
Claudete S. Astolfi-Ferreira9
Estimated H-index: 9
(USP: University of São Paulo)
+ 6 AuthorsAntonio José Piantino Ferreira24
Estimated H-index: 24
(USP: University of São Paulo)
Abstract
1. Infectious bronchitis virus (IBV) variants in Brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. 2. Of the 453 samples examined, 61.4% were positive for IBV and 75.9% of these were considered to have the BR-I genotype and were detected in birds of all ages distributed in all five Brazilian regions. 3. The ratio of non-synonymous substitutions per non-synonymous site (dN) to synonymous substitutions per synonymous site (dS), i.e. dN/dS, revealed a predominance of codons with non-synonymous substitutions in the first third of the S1 gene and a dN/dS ratio of 0.67. Additionally, prediction of N-glycosylation sites showed that most of the BR-I variants (from 2003 to early 2014) had an extra site at amino acid position 20, whereas the newest variants lacked this extra site. 4. These results suggest that Brazilian IBV variants probably underwent drastic mutations at various points between 1983 and 2003 and that the selection processes became silent after achieving a sufficiently effective antigenic structure for invasion and replication in their hosts. Brazilian IBV genotype BR-I is currently the predominant genotype circulating in Brazil and South America.
  • References (50)
  • Citations (3)
📖 Papers frequently viewed together
17 Citations
31 Citations
7 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References50
Newest
#1Viviana Valastro (UNIPD: University of Padua)H-Index: 1
#1Viviana Valastro (UNIPD: University of Padua)H-Index: 9
Last. Isabella MonneH-Index: 27
view all 7 authors...
Infectious bronchitis virus (IBV) is the causative agent of a highly contagious disease that results in severe economic losses to the global poultry industry. The virus exists in a wide variety of genetically distinct viral types, and both phylogenetic analysis and measures of pairwise similarity among nucleotide or amino acid sequences have been used to classify IBV strains. However, there is currently no consensus on the method by which IBV sequences should be compared, and heterogeneous genet...
128 CitationsSource
#1Joeri Kint (WUR: Wageningen University and Research Centre)H-Index: 5
#2Annemiek Dickhout (WUR: Wageningen University and Research Centre)H-Index: 1
Last. Maria Forlenza (WUR: Wageningen University and Research Centre)H-Index: 24
view all 10 authors...
The innate immune response is the first line of defense against viruses, and type I interferon (IFN) is a critical component of this response. Similar to other viruses, the gammacoronavirus infectious bronchitis virus (IBV) has evolved under evolutionary pressure to evade and counteract the IFN response to enable its survival. Previously, we reported that IBV induces a delayed activation of the IFN response. In the present work, we describe the resistance of IBV to IFN and the potential role of ...
22 CitationsSource
#1Ana MarandinoH-Index: 7
#2Ariel Pereda (INTA: International Trademark Association)H-Index: 18
Last. Ruben PérezH-Index: 26
view all 11 authors...
Infectious bronchitis virus (IBV) is a coronavirus of chickens that causes great economic losses to the global poultry industry. The present study focuses on South American IBVs and their genetic relationships with global strains. We obtained full-length sequences of the S1 coding region and N gene of IBV field isolates from Uruguay and Argentina, and performed Phylodynamic analysis to characterize the strains and estimate the time of the most recent common ancestor. We identified two major Sout...
28 CitationsSource
#1I. N. Ambepitiya Wickramasinghe (UU: Utrecht University)H-Index: 5
#2S. J. van Beurden (UU: Utrecht University)H-Index: 3
Last. Monique H. Verheije (UU: Utrecht University)H-Index: 22
view all 4 authors...
Abstract Avian coronaviruses of the genus Gammacoronavirus are represented by infectious bronchitis virus (IBV), the coronavirus of chicken. IBV causes a highly contagious disease affecting the respiratory tract and, depending on the strain, other tissues including the reproductive and urogenital tract. The control of IBV in the field is hampered by the many different strains circulating worldwide and the limited protection across strains due to serotype diversity. This diversity is believed to ...
40 CitationsSource
#1Benjamin W. Neuman (University of Reading)H-Index: 24
#2P. G. Chamberlain (University of Reading)H-Index: 8
Last. Jeremiah S. JosephH-Index: 18
view all 4 authors...
The international response to SARS-CoV has produced an outstanding number of protein structures in a very short time. This review summarizes the findings of functional and structural studies including those derived from cryoelectron microscopy, small angle X-ray scattering, NMR spectroscopy, and X-ray crystallography, and incorporates bioinformatics predictions where no structural data is available. Structures that shed light on the function and biological roles of the proteins in viral replicat...
28 CitationsSource
#1Eder Balestrin (ULBRA: Universidade Luterana do Brasil)H-Index: 3
#2Aline Padilha de Fraga (ULBRA: Universidade Luterana do Brasil)H-Index: 3
Last. Vagner Ricardo Lunge (ULBRA: Universidade Luterana do Brasil)H-Index: 12
view all 6 authors...
Abstract Avian infectious bronchitis is a highly contagious viral disease with economic effects on poultry agribusiness. The disease presents multi-systemic clinical signs (respiratory, renal, enteric, and reproductive) and is caused by one coronavirus (infectious bronchitis virus, IBV). Infectious bronchitis virus is classified into different serotypes and genotypes (vaccine strains and field variants). This study aimed to evaluate the occurrence of IBV in commercial poultry flocks from 3 impor...
16 CitationsSource
#1Jorge Luis Chacón (USP: University of São Paulo)H-Index: 9
#2M. S. Assayag (USP: University of São Paulo)H-Index: 3
Last. A. J. Piantino Ferreira (USP: University of São Paulo)H-Index: 6
view all 7 authors...
Abstract1. The possibility that infectious bronchitis virus (IBV) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those IBV strains causing classical respiratory disease was investigated.2. IBV variants were isolated from broiler flocks with enteric and respiratory problems in two regions of Brazil. The USP-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into IBV-antibody-free broilers and...
13 CitationsSource
#1Koichiro Tamura (Tokyo Metropolitan University)H-Index: 31
#2Glen Stecher (ASU: Arizona State University)H-Index: 8
Last. Sudhir Kumar (ASU: Arizona State University)H-Index: 68
view all 5 authors...
We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference...
26.7k CitationsSource
#1R. J. de Groot (UU: Utrecht University)H-Index: 20
#1Raoul J. de Groot (UU: Utrecht University)H-Index: 28
Last. John Ziebuhr (University of Giessen)H-Index: 41
view all 18 authors...
During the summer of 2012, in Jeddah, Saudi Arabia, a hitherto unknown coronavirus (CoV) was isolated from the sputum of a patient with acute pneumonia and renal failure ([1][1], [2][2]). The isolate was provisionally called human coronavirus Erasmus Medical Center (EMC) ([3][3]). Shortly thereafter
490 CitationsSource
#1Beth N. Licitra (Cornell University)H-Index: 6
#2Jean K. Millet (Université Paris-Saclay)H-Index: 14
Last. Gary R. Whittaker (Cornell University)H-Index: 39
view all 7 authors...
Feline coronaviruses (FCoV) exist as 2 biotypes: feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV). FECV causes subclinical infections; FIPV causes feline infectious peritonitis (FIP), a systemic and fatal disease. It is thought that mutations in FECV enable infection of macrophages, causing FIP. However, the molecular basis for this biotype switch is unknown. We examined a furin cleavage site in the region between receptor-binding (S1) and fusion (S2) domains of t...
77 CitationsSource
Cited By3
Newest
#1Ruy D. Chacón (USP: University of São Paulo)H-Index: 2
#2Claudete S. Astolfi-Ferreira (USP: University of São Paulo)H-Index: 9
Last. Antonio José Piantino Ferreira (USP: University of São Paulo)H-Index: 24
view all 6 authors...
Abstract Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positiv...
Source
#1Wen-Sheng Fan (Xida: Guangxi University)H-Index: 4
#2Ning Tang (Xida: Guangxi University)H-Index: 2
Last. Ping WeiH-Index: 9
view all 13 authors...
The high mutation rates of infectious bronchitis virus (IBV) pose economic threats to the poultry industry. In order to track the genetic evolutionary of IBV isolates circulating in yellow chickens, we continued to conduct the genetic analyses of the structural genes S1, E, M, and N from 64 IBV isolates in southern China during 2009–2017. The results showed that the dominant genotypes based on the four genes had changed when compared with those during 1985–2008. Based on the S1 gene phylogenetic...
5 CitationsSource
#1Aline Padilha de Fraga (ULBRA: Universidade Luterana do Brasil)H-Index: 3
#2Tiago Gräf (UKZN: University of KwaZulu-Natal)H-Index: 3
Last. Vagner Ricardo Lunge (ULBRA: Universidade Luterana do Brasil)H-Index: 12
view all 6 authors...
Abstract Avian infectious bronchitis virus (IBV) is the etiological agent of a highly contagious disease, which results in severe economic losses to the poultry industry. The spike protein (S1 subunit) is responsible for the molecular diversity of the virus and many sero/genotypes are described around the world. Recently a new standardized classification of the IBV molecular diversity was conducted, based on phylogenetic analysis of the S1 gene sequences sampled worldwide. Brazil is one of the b...
3 CitationsSource