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Genetic Characterisation and Analysis of Infectious Bronchitis Virus Isolated from Brazilian flocks between 2010 and 2015.

Published on Nov 2, 2017in British Poultry Science1.42
· DOI :10.1080/00071668.2017.1365116
Claudia Carranza3
Estimated H-index: 3
(USP: University of São Paulo),
Claudete S. Astolfi-Ferreira7
Estimated H-index: 7
(USP: University of São Paulo)
+ 6 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
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Abstract
1. Infectious bronchitis virus (IBV) variants in Brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. 2. Of the 453 samples examined, 61.4% were positive for IBV and 75.9% of these were considered to have the BR-I genotype and were detected in birds of all ages distributed in all five Brazilian regions. 3. The ratio of non-synonymous substitutions per non-synonymous site (dN) to synonymous substitutions per synonymous site (dS), i.e. dN/dS, revealed a predominance of codons with non-synonymous substitutions in the first third of the S1 gene and a dN/dS ratio of 0.67. Additionally, prediction of N-glycosylation sites showed that most of the BR-I variants (from 2003 to early 2014) had an extra site at amino acid position 20, whereas the newest variants lacked this extra site. 4. These results suggest that Brazilian IBV variants probably underwent drastic mutations at various points between 1983 and 2003 and that the selection processes became silent after achieving a sufficiently effective antigenic structure for invasion and replication in their hosts. Brazilian IBV genotype BR-I is currently the predominant genotype circulating in Brazil and South America.
  • References (50)
  • Citations (1)
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References50
Newest
Published on Apr 1, 2016in Infection, Genetics and Evolution2.61
Viviana Valastro9
Estimated H-index: 9
(UNIPD: University of Padua),
Edward C. Holmes109
Estimated H-index: 109
(USYD: University of Sydney)
+ 4 AuthorsIsabella Monne24
Estimated H-index: 24
Infectious bronchitis virus (IBV) is the causative agent of a highly contagious disease that results in severe economic losses to the global poultry industry. The virus exists in a wide variety of genetically distinct viral types, and both phylogenetic analysis and measures of pairwise similarity among nucleotide or amino acid sequences have been used to classify IBV strains. However, there is currently no consensus on the method by which IBV sequences should be compared, and heterogeneous genet...
Published on Dec 1, 2015in Journal of Virology4.32
Joeri Kint5
Estimated H-index: 5
(WUR: Wageningen University and Research Centre),
Annemiek Dickhout1
Estimated H-index: 1
(WUR: Wageningen University and Research Centre)
+ 7 AuthorsM. Forlenza23
Estimated H-index: 23
(WUR: Wageningen University and Research Centre)
The innate immune response is the first line of defense against viruses, and type I interferon (IFN) is a critical component of this response. Similar to other viruses, the gammacoronavirus infectious bronchitis virus (IBV) has evolved under evolutionary pressure to evade and counteract the IFN response to enable its survival. Previously, we reported that IBV induces a delayed activation of the IFN response. In the present work, we describe the resistance of IBV to IFN and the potential role of ...
Published on Jun 1, 2015in Journal of General Virology2.81
Ana Marandino5
Estimated H-index: 5
,
Ariel Pereda17
Estimated H-index: 17
(INTA: International Trademark Association)
+ 8 AuthorsYanina Panzera15
Estimated H-index: 15
Infectious bronchitis virus (IBV) is a coronavirus of chickens that causes great economic losses to the global poultry industry. The present study focuses on South American IBVs and their genetic relationships with global strains. We obtained full-length sequences of the S1 coding region and N gene of IBV field isolates from Uruguay and Argentina, and performed Phylodynamic analysis to characterize the strains and estimate the time of the most recent common ancestor. We identified two major Sout...
Published on Dec 1, 2014in Virus Research2.74
Benjamin W. Neuman20
Estimated H-index: 20
(University of Reading),
P. G. Chamberlain8
Estimated H-index: 8
(University of Reading)
+ 1 AuthorsJeremiah S. Joseph17
Estimated H-index: 17
The international response to SARS-CoV has produced an outstanding number of protein structures in a very short time. This review summarizes the findings of functional and structural studies including those derived from cryoelectron microscopy, small angle X-ray scattering, NMR spectroscopy, and X-ray crystallography, and incorporates bioinformatics predictions where no structural data is available. Structures that shed light on the function and biological roles of the proteins in viral replicat...
Published on Dec 1, 2014in Virus Research2.74
I. N. Ambepitiya Wickramasinghe5
Estimated H-index: 5
(UU: Utrecht University),
S. J. van Beurden4
Estimated H-index: 4
(UU: Utrecht University)
+ 1 AuthorsMonique H. Verheije21
Estimated H-index: 21
(UU: Utrecht University)
Abstract Avian coronaviruses of the genus Gammacoronavirus are represented by infectious bronchitis virus (IBV), the coronavirus of chicken. IBV causes a highly contagious disease affecting the respiratory tract and, depending on the strain, other tissues including the reproductive and urogenital tract. The control of IBV in the field is hampered by the many different strains circulating worldwide and the limited protection across strains due to serotype diversity. This diversity is believed to ...
Published on Aug 1, 2014in Poultry Science2.03
Eder Balestrin3
Estimated H-index: 3
(ULBRA: Universidade Luterana do Brasil),
Aline Padilha Fraga3
Estimated H-index: 3
(ULBRA: Universidade Luterana do Brasil)
+ 3 AuthorsVagner Ricardo Lunge12
Estimated H-index: 12
(ULBRA: Universidade Luterana do Brasil)
Published on May 4, 2014in British Poultry Science1.42
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
M. S. Assayag3
Estimated H-index: 3
(USP: University of São Paulo)
+ 4 AuthorsA. J. Piantino Ferreira6
Estimated H-index: 6
(USP: University of São Paulo)
Abstract1. The possibility that infectious bronchitis virus (IBV) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those IBV strains causing classical respiratory disease was investigated.2. IBV variants were isolated from broiler flocks with enteric and respiratory problems in two regions of Brazil. The USP-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into IBV-antibody-free broilers and...
Published on Dec 1, 2013in Molecular Biology and Evolution14.80
Koichiro Tamura28
Estimated H-index: 28
(Tokyo Metropolitan University),
Glen Stecher6
Estimated H-index: 6
(ASU: Arizona State University)
+ 2 AuthorsSudhir Kumar63
Estimated H-index: 63
(ASU: Arizona State University)
We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference...
Published on Jul 1, 2013in Emerging Infectious Diseases7.18
Beth N. Licitra6
Estimated H-index: 6
,
Jean K. Millet8
Estimated H-index: 8
+ 4 AuthorsGary R. Whittaker38
Estimated H-index: 38
Feline coronaviruses (FCoV) exist as 2 biotypes: feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV). FECV causes subclinical infections; FIPV causes feline infectious peritonitis (FIP), a systemic and fatal disease. It is thought that mutations in FECV enable infection of macrophages, causing FIP. However, the molecular basis for this biotype switch is unknown. We examined a furin cleavage site in the region between receptor-binding (S1) and fusion (S2) domains of t...
Published on Jun 1, 2013in Avian Diseases1.31
Ha-Jung Roh4
Estimated H-index: 4
,
Deborah A. Hilt23
Estimated H-index: 23
+ 1 AuthorsMark W. Jackwood32
Estimated H-index: 32
SUMMARY Infectious bronchitis virus (IBV) causes an upper respiratory tract disease in chickens and is highly contagious. Many different types of the virus exist, but only a few types are used as attenuated live vaccines in the commercial poultry industry. Of the vaccine types used, the Arkansas (Ark)–type virus is most frequently reisolated from vaccinated broilers. Previous research has suggested that incomplete clearance of Ark-type vaccine virus plays a role in the inadequate protection obse...
Cited By1
Newest
Published on 2019in Molecular and Cellular Probes2.51
Ruy D. Chacón1
Estimated H-index: 1
(USP: University of São Paulo),
Claudete S. Astolfi-Ferreira7
Estimated H-index: 7
(USP: University of São Paulo)
+ 3 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Abstract Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positiv...
Published on 2019in Viruses3.81
Wen-Sheng Fan3
Estimated H-index: 3
,
Ning Tang + 7 AuthorsTianchao Wei5
Estimated H-index: 5
The high mutation rates of infectious bronchitis virus (IBV) pose economic threats to the poultry industry. In order to track the genetic evolutionary of IBV isolates circulating in yellow chickens, we continued to conduct the genetic analyses of the structural genes S1, E, M, and N from 64 IBV isolates in southern China during 2009–2017. The results showed that the dominant genotypes based on the four genes had changed when compared with those during 1985–2008. Based on the S1 gene phylogenetic...
Published on Jul 1, 2018in Infection, Genetics and Evolution2.61
Aline Padilha Fraga3
Estimated H-index: 3
(ULBRA: Universidade Luterana do Brasil),
Aline Padilha de Fraga1
Estimated H-index: 1
+ 3 AuthorsVagner Ricardo Lunge12
Estimated H-index: 12
(ULBRA: Universidade Luterana do Brasil)
Abstract Avian infectious bronchitis virus (IBV) is the etiological agent of a highly contagious disease, which results in severe economic losses to the poultry industry. The spike protein (S1 subunit) is responsible for the molecular diversity of the virus and many sero/genotypes are described around the world. Recently a new standardized classification of the IBV molecular diversity was conducted, based on phylogenetic analysis of the S1 gene sequences sampled worldwide. Brazil is one of the b...
View next paperEpidemiological survey and molecular characterization of avian infectious bronchitis virus in Brazil between 2003 and 2009