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  • Citations (6)
Published on Aug 1, 2017in Nature 41.58
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Nuria Marti-Gutierrez1
Estimated H-index: 1
(Oregon Health & Science University)
+ 28 AuthorsRiffat Ahmed10
Estimated H-index: 10
(Oregon Health & Science University)
CRISPR–Cas9 genome editing is used to induce a DNA repair response and correct a disease-causing heterozygous mutation in human embryos with reduced mosaicism and preferential repair using the wild-type copy of the gene.
259 Citations Source Cite
Published on Jun 1, 2017in Molecular Genetics and Genomics 2.73
Lichun Tang1
Estimated H-index: 1
(Protein Sciences),
Yanting Zeng1
Estimated H-index: 1
(Guangzhou Medical University)
+ 8 AuthorsXiaowei Li1
Estimated H-index: 1
(Nanjing University)
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into o...
80 Citations Source Cite
Published on May 1, 2015in Scientific Reports 4.12
Toru Suzuki6
Estimated H-index: 6
Maki Asami3
Estimated H-index: 3
Anthony C.F. Perry33
Estimated H-index: 33
Mammalian genomes can be edited by injecting pronuclear embryos with Cas9 cRNA and guide RNA (gRNA) but it is unknown whether editing can also occur during the onset of embryonic development, prior to pronuclear embryogenesis. We here report Cas9-mediated editing during sperm-induced meiotic exit and the initiation of development. Injection of unfertilized, mouse metaphase II (mII) oocytes with Cas9 cRNA, gRNA and sperm enabled efficient editing of transgenic and native alleles. Pre-loading oocy...
24 Citations Source Cite
Cited By6
Published on Jan 1, 2019
Sonia Ben Ouagrham-Gormley4
Estimated H-index: 4
(George Mason University),
Shannon R. Fye-Marnien1
Estimated H-index: 1
Since 2012, the new gene editing technique called CRISPR took the world by storm because theoretically it can be used to edit any organism quickly, precisely, and at low cost. Because of these features, many fear that CRISPR could become a technology of choice for terrorists or states who wish to produce novel threat agents or bioweapons. Others fear that it could be the source of a catastrophic event caused by unsafe laboratory practices by amateur or practicing scientists. In this chapter we r...
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Published on Jan 1, 2019in Molecular Neurobiology 5.08
Sudhanshu P. Raikwar14
Estimated H-index: 14
(University of Missouri),
Ramasamy Thangavel6
Estimated H-index: 6
(University of Missouri)
+ 6 AuthorsAsgar Zaheer29
Estimated H-index: 29
(University of Missouri)
Alzheimer’s disease (AD) is a devastating, progressive neurodegenerative disorder that leads to severe cognitive impairment in elderly patients. Chronic neuroinflammation plays an important role in the AD pathogenesis. Glia maturation factor (GMF), a proinflammatory molecule discovered in our laboratory, is significantly upregulated in various regions of AD brains. We have previously reported that GMF is predominantly expressed in the reactive glial cells surrounding the amyloid plaques (APs) in...
5 Citations Source Cite
Published on Jan 1, 2019in Progress in Retinal and Eye Research 11.65
Jia Hui Lee3
Estimated H-index: 3
Jiang-Hui Wang5
Estimated H-index: 5
(University of Melbourne)
+ 4 AuthorsGuei-Sheung Liu18
Estimated H-index: 18
Abstract Many clinical trials using gene therapy have shown significant therapeutic benefits and exceptional safety records. Increasing evidence is verifying the long sought-after promise that gene therapy will genetically ‘cure’ some severely disabling diseases. In particular, the first gene therapy bioproduct for RPE65 -associated Leber's congenital amaurosis, which was approved by the US Food and Drug Administration in 2017, has provided tremendous encouragement to the field of gene therapy. ...
5 Citations Source Cite
Published on Oct 1, 2018in Hearing Research 2.82
Jenna Devare1
Estimated H-index: 1
(Kresge Hearing Research Institute),
Samuel P. Gubbels11
Estimated H-index: 11
(University of Colorado Denver),
Yehoash Raphael50
Estimated H-index: 50
(Kresge Hearing Research Institute)
Abstract Drug delivery to the inner ear is an ideal method to treat a wide variety of otologic conditions. A broad range of potential applications is just beginning to be explored. New approaches combine principles of inner ear pharmacokinetics with emerging technologies of drug delivery including novel delivery systems, drug-device combinations, and new categories of drugs. Strategies include cell-specific targeting, manipulation of gene expression, local activation following systemic delivery,...
3 Citations Source Cite
Published on Jan 1, 2018
Karsten Schürrle2
Estimated H-index: 2
The past 150 years have seen remarkable discoveries, rapidly growing biological knowledge, and giant technological leaps providing biotechnological solutions for healthcare, food production, and other societal needs. Genetic engineering, miniaturization, and ever-increasing computing power, in particular, have been key technological drivers for the past few decades. Looking ahead, the eventual transition from fossil resources to biomass and CO2 demands a shift toward a ‘bio-economy’ based on nov...
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