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Functional Characterization of TaSnRK2.8 Promoter in Response to Abiotic Stresses by Deletion Analysis in Transgenic Arabidopsis

Published on Jul 13, 2017in Frontiers in Plant Science4.106
· DOI :10.3389/fpls.2017.01198
Hongying Zhang5
Estimated H-index: 5
(Henan Agricultural University),
Ruilian Jing23
Estimated H-index: 23
,
Xinguo Mao4
Estimated H-index: 4
Abstract
Drought, salinity, and cold are the major factors limiting wheat quality and productivity; it is thus highly desirable to characterise the abiotic-stress-inducible promoters suitable for the genetic improvement of plant resistance. The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family genes show distinct regulatory properties in response to abiotic stresses. The present study characterized the approximately 3000-bp upstream sequence (the 313 bp upstream of the ATG was the transcription start site) of the Triticum aestivum TaSnRK2.8 promoter under abscisic acid (ABA) and abiotic stresses. Four different-length 5′ deletion fragments of TaSnRK2.8 promoter were fused with the GUS reporter gene and transformed into Arabidopsis. Tissue expression analysis showed that the TaSnRK2.8 promoter region from position −1481 to −821 contained the stalk-specific elements, and the region from position −2631 to −1481 contained the leaf- and root-specific elements. In the ABA-treated seedlings, the deletion analysis showed that the TaSnRK2.8 promoter region from position −821 to −2631 contained ABA response elements. The abiotic stress responses of the TaSnRK2.8 promoter derivatives demonstrated that they harboured abiotic-stress response elements: the region from position −821 to −408 harboured the osmotic-stress response elements, whereas the region from position −2631 to −1481 contained the positive regulatory motifs and the region from position −1481 to −821 contained the leaf- and stalk-specific enhancers. Further deletion analysis of the promoter region from position −821 to −408 indicated that a 125-bp region from position −693 to −568 was required to induce an osmotic-stress response. These results contribute to a better understanding of the molecular mechanisms of TaSnRK2.8 in response to abiotic stresses, and the TaSnRK2.8 promoter seems to be a candidate for regulating the expression of abiotic stress response genes in transgenic plants.
  • References (34)
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References34
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#1Jiajia Hou (SDU: Shandong University)H-Index: 3
#2Pingping Jiang (SDU: Shandong University)H-Index: 2
Last. Kunpeng Li (SDU: Shandong University)H-Index: 9
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Salinity and drought severely affect both plant growth and productivity, making the isolation and characterization of salinity- or drought-inducible promoters suitable for genetic improvement of crop resistance highly desirable. In this study, a 1468-bp sequence upstream of the translation initiation codon ATG of the promoter for ZmGAPP (maize Type-II H+-pyrophosphatase gene) was cloned. Nine 5´ deletion fragments (D1–D9) of different lengths of the ZmGAPP promoter were fused with the GUS report...
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#1Hongying Zhang (Henan Agricultural University)H-Index: 5
#2Weiyu Li (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 2
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Plant responses to stress occur via abscisic acid (ABA) dependent or independent pathways. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) play a key role in plant stress signal transduction pathways. It is known that some SnRK2 members are positive regulators of ABA signal transduction through interaction with group A type 2C protein phosphatases (PP2Cs). Here, ten SnRK2s were isolated from wheat. Based on phylogenetic analysis using kinase domains or the C-terminus, the ten SnRK2s wer...
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#1Hongying Zhang (Henan Agricultural University)H-Index: 5
#2Xinguo MaoH-Index: 1
Last. Ruilian JingH-Index: 23
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Abstract TaSnRK2.8, an SnRK2 (sucrose non-fermenting1-related protein kinase 2) member of wheat, confers enhanced multi-stress tolerances in carbohydrate metabolism. In the study, two types of genomic sequences of TaSnRK2.8 were detected in common wheat. Sequencing analysis showed that there was a variation-enriched region, designated TaSnRK2.8-A-C, covering the eighth intron, the ninth exon and the 3′-flanking region of TaSnRK2.8-A, and no divergence occurred in TaSnRK2.8-B. Single nucleotide po...
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