Genetically encoded calcium indicators for fluorescence imaging in the moss Physcomitrella: GCaMP3 provides a bright new look

The moss Physcomitrella patens is emerging as a model organism due to the availability of established protocols for precise genome engineering by homologous recombination and a high-quality genome sequence (Rensing et al., 2008). Physcomitrella is an attractive system for cell biology and live cell imaging and has been used to investigate fundamental cellular processes such as tip growth, vesicle trafficking and cytoskeletal dynamics (e.g., Furt...