Analytical shortcomings and other considerations related to the identification of biomarkers of dairy fat intake

Over the last decade, the concentrations of certain odd-chain saturated fatty acids (for example, C15:0 and C17:0) and trans-palmitoleic acid (trans-9 C16:1) in plasma and erythrocytes have been proposed as biomarkers of dairy fat intake,1, 2, 3 which is expected to overcome major issues associated with dietary assessment methods that rely on self-report, such as food frequency questionnaires. However, some overlooked analytical issues may have...