Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

Nature64.80
Volume: 550, Issue: 7676, Pages: 407 - 410
Published: Sep 20, 2017
Abstract
The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing. High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human cells, but the mechanism of target discrimination and the potential to further improve fidelity are unknown. Here, using single-molecule Forster resonance energy transfer experiments, we show...
Paper Details
Title
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
Published Date
Sep 20, 2017
Journal
Volume
550
Issue
7676
Pages
407 - 410
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