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In vivo analysis of mucosal lipids reveals histological disease activity in ulcerative colitis using endoscope-coupled Raman spectroscopy

Published on Jul 1, 2017in Biomedical Optics Express3.91
· DOI :10.1364/BOE.8.003426
Hao Ding6
Estimated H-index: 6
(University of Texas Health Science Center at Houston),
Andrew W. DuPont11
Estimated H-index: 11
(University of Texas Health Science Center at Houston)
+ 4 AuthorsXiaohong Bi17
Estimated H-index: 17
(University of Texas Health Science Center at Houston)
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Abstract
The goal of this study is to evaluate endoscopic Raman spectroscopy as a noninvasive technique to determine histological inflammatory status of colitis. Colon mucosal composition was investigated in vivo from patients with ulcerative colitis (UC) and from age- and body mass index (BMI) matched controls using endoscope-coupled Raman spectroscopy. The results were co-registered with histological assessment of inflammatory status at the same locations. Substantial decreases (50-60%) in the content of phosphotidylcholines (PCs) and total lipids were observed in inflamed colon tissue (histology grade 1, 2 and 3) compared to those from the quiescent (histology grade 0) and from the controls. No significant difference was observed in lipids or PC contents between control and grade 0, or among grades 1 – 3. The degree of lipid unsaturation increased in the inflamed tissue regardless of disease severity. The inflammation-associated alterations in lipids and PC are observed independent of BMI or the anatomical locations for data collection. Multivariate analysis using support vector machine (SVM) algorithm classified the spectra of the controls or the inactive colitis from those of inflamed tissue with a sensitivity of 83.5% and 97.1% respectively. Our results showed that mucosal lipid content is related to the microscopic disease activity, and thus could serve as a valuable spectral marker to differentiate active colitis from the quiescent.
  • References (50)
  • Citations (5)
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References50
Newest
Published on Feb 1, 2017in Biomedical Optics Express3.91
Isaac J. Pence7
Estimated H-index: 7
(Vandy: Vanderbilt University),
Dawn B. Beaulieu11
Estimated H-index: 11
(Vandy: Vanderbilt University)
+ 4 AuthorsAnita Mahadevan-Jansen38
Estimated H-index: 38
(Vandy: Vanderbilt University)
Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectrosc...
Published on Feb 1, 2017in Scientific Reports4.01
Krzysztof Czamara6
Estimated H-index: 6
,
Katarzyna Majzner11
Estimated H-index: 11
+ 3 AuthorsAgnieszka Kaczor15
Estimated H-index: 15
Unsaturated lipid bodies as a hallmark of inflammation studied by Raman 2D and 3D microscopy
Published on May 1, 2016in Biomedical Optics Express3.91
James Addis1
Estimated H-index: 1
(University of Leeds),
Noor Mohammed3
Estimated H-index: 3
(University of Leeds)
+ 3 AuthorsVenkataraman Subramanian22
Estimated H-index: 22
(University of Leeds)
Raman spectroscopy was used to differentiate between mucosally healed (or quiescent) and inflamed colon tissue, as assessed endoscopically, in patients with ulcerative colitis. From the analysis of the Raman spectra of 60 biopsy tissue samples, clear differences were identified between the spectra of the quiescent and inflamed tissue. Three carotenoid peaks were found to be approximately twice as intense in the inflamed tissue. Two phospholipid peaks were found to be significantly lower in the i...
Published on Apr 1, 2016in Journal of Biophotonics3.76
Mads Sylvest Bergholt16
Estimated H-index: 16
(NUS: National University of Singapore),
Kan Lin13
Estimated H-index: 13
(NUS: National University of Singapore)
+ 10 AuthorsSupriya Srivastava15
Estimated H-index: 15
(NUS: National University of Singapore)
Published on Mar 7, 2016in Proceedings of SPIE
Hao Ding6
Estimated H-index: 6
(University of Texas at Austin),
Ming Cao1
Estimated H-index: 1
(University of Texas at Austin)
+ 10 AuthorsDavid Schwartz26
Estimated H-index: 26
(Vandy: Vanderbilt University)
Inflammatory bowel disease (IBD) is an idiopathic disease that is typically characterized by chronic inflammation of the gastrointestinal tract. Recently much effort has been devoted to the development of novel diagnostic tools that can assist physicians for fast, accurate, and automated diagnosis of the disease. Previous research based on Raman spectroscopy has shown promising results in differentiating IBD patients from normal screening cases. In the current study, we examined IBD patients in ...
Published on Feb 1, 2016in European Journal of Immunology4.70
Marina Rosengarten1
Estimated H-index: 1
(BGU: Ben-Gurion University of the Negev),
Nurit Hadad15
Estimated H-index: 15
(BGU: Ben-Gurion University of the Negev)
+ 2 AuthorsRachel Levy31
Estimated H-index: 31
(BGU: Ben-Gurion University of the Negev)
Colitis, an inflammation of the colon, is a well-characterized massive tissue injury. Cytosolic phospholipase A2 α (cPLA2 α) upregulation plays an important role in the development of several inflammatory diseases. The aim of the present study was to define the role of cPLA2 α upregulation in the development of colitis. We used a mouse model of dextran sulfate sodium induced colitis. Immunoblotting analysis showed that cPLA2 α and NF-κB were upregulated and activated in the colon from day 2 of c...
Published on Jan 1, 2016in Journal of Biophotonics3.76
Michael A. Short11
Estimated H-index: 11
(BC Cancer Agency),
Wenbo Wang4
Estimated H-index: 4
(BC Cancer Agency)
+ 1 AuthorsHaishan Zeng7
Estimated H-index: 7
(BC Cancer Agency)
The objective of this study was to build and test an adjunct system to a colonoscope for in vivo measurement of Raman spectra from colon tissue for potentially improving the detection of early cancers. The novelty of this system was that low cost fibre optic probes were used, without the addition of expensive optical filters. Good quality in vivo Raman spectra were successfully obtained with a 1 s integration time in the high frequency (HF) range from normal tissue and polyps of patients during ...
Marcello Cintolo1
Estimated H-index: 1
,
Giuseppe Costantino12
Estimated H-index: 12
+ 1 AuthorsWalter Fries28
Estimated H-index: 28
In the past decade, thanks to the introduction of biologic therapies, a new therapeutic goal, mucosal healing (MH), has been introduced. MH is the expression of an arrest of disease progression, resulting in minor hospitalizations, surgeries, and prolonged clinical remission. MH may be achieved with several therapeutic strategies reaching success rates up to 80% for both, ulcerative colitis (UC) and Crohn’s disease (CD). Various scoring systems for UC and for the transmural CD, have been propose...
Published on Feb 1, 2015in Clinics and Research in Hepatology and Gastroenterology2.81
AntonioTursi35
Estimated H-index: 35
,
Walter Elisei17
Estimated H-index: 17
+ 5 AuthorsGiovanni Brandimarte22
Estimated H-index: 22
Summary Background and objective There are few data on how histological signs of inflammation develop under treatment with infliximab (IFX). We investigated the patterns of histological features of inflammation in patients with UC in sustained clinical and endoscopic remission under IFX. Methods We performed a retrospective study on 47 patients with UC in clinical and endoscopic remission and undergoing surveillance colonoscopy with biopsies while receiving maintenance therapy with IFX. Each col...
Published on Jan 20, 2015in Analytical Chemistry6.35
Mads Sylvest Bergholt16
Estimated H-index: 16
,
Wei Zheng29
Estimated H-index: 29
+ 7 AuthorsZhiwei Huang35
Estimated H-index: 35
This study aims to characterize the in vivo Raman spectroscopic properties of normal colorectal tissues and to assess distinctive biomolecular variations of different anatomical locations in the colorectum for cancer diagnosis. We have developed a novel 785 nm excitation fiber-optic Raman endoscope that can simultaneously acquire in vivo fingerprint (FP) spectra (800–1800 cm–1) and high-wavenumber (HW) Raman spectra (2800–3600 cm–1) from the subsurface of colorectal tissue. We applied the FP/HW ...
Cited By5
Newest
Published on Feb 17, 2019in Inflammatory Bowel Diseases4.00
Urszula Daniluk2
Estimated H-index: 2
(UMB: Medical University of Białystok),
Jaroslaw Daniluk9
Estimated H-index: 9
(UMB: Medical University of Białystok)
+ 7 AuthorsMichal Ciborowski13
Estimated H-index: 13
(UMB: Medical University of Białystok)
Metabolic profiling might be used to identify disease biomarkers. The aim of our study was to determine the usefulness of untargeted metabolomics analysis to detect differences in serum metabolites between newly diagnosed and untreated pediatric patients with Crohn’s disease (CD) or ulcerative colitis (UC) in comparison with a control group (Ctr). Moreover, we investigated the potential of profiling metabolomics and inflammatory markers to improve the noninvasive diagnosis of CD and UC in childr...
Published on Dec 1, 2018in Journal of Food Biochemistry1.36
Danfei Huang15
Estimated H-index: 15
(NCU: Nanchang University),
Qiang Xia (NCU: Nanchang University)+ 3 AuthorsMingyong Xie40
Estimated H-index: 40
(NCU: Nanchang University)
Published on Dec 1, 2018in Light-Science & Applications14.00
Alberto Lombardini2
Estimated H-index: 2
(AMU: Aix-Marseille University),
Vasyl Mytskaniuk2
Estimated H-index: 2
(AMU: Aix-Marseille University)
+ 8 AuthorsAlexandre Kudlinski20
Estimated H-index: 20
(university of lille)
Coherent Raman scattering microscopy is a fast, label-free, and chemically specific imaging technique that shows high potential for future in vivo optical histology. However, the imaging depth in tissues is limited to the sub-millimeter range because of absorption and scattering. Realization of coherent Raman imaging using a fiber endoscope system is a crucial step towards imaging deep inside living tissues and providing information that is inaccessible with current microscopy tools. Until now, ...
Published on Jun 28, 2018in Journal of Biomedical Optics2.56
Eliana Cordero2
Estimated H-index: 2
,
Ines Latka14
Estimated H-index: 14
+ 2 AuthorsJürgen Popp59
Estimated H-index: 59
(FSU: University of Jena)
For more than two decades, Raman spectroscopy has found widespread use in biological and medical applications. The instrumentation and the statistical evaluation procedures have matured, enabling the lengthy transition from ex-vivo demonstration to in-vivo examinations. This transition goes hand-in-hand with many technological developments and tightly bound requirements for a successful implementation in a clinical environment, which are often difficult to assess for novice scientists in the fie...
Published on Jan 1, 2018in RSC Advances3.05
Nikki Kuhar1
Estimated H-index: 1
(IISc: Indian Institute of Science),
Sanchita Sil5
Estimated H-index: 5
(DRDO: Defence Research and Development Organisation)
+ 1 AuthorsSiva Umapathy22
Estimated H-index: 22
(IISc: Indian Institute of Science)
Raman spectroscopy has become an essential tool for chemists, physicists, biologists and materials scientists. In this article, we present the challenges in unravelling the molecule-specific Raman spectral signatures of different biomolecules like proteins, nucleic acids, lipids and carbohydrates based on the review of our work and the current trends in these areas. We also show how Raman spectroscopy can be used to probe the secondary and tertiary structural changes occurring during thermal den...
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