Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production inPichia pastoris

Volume: 114, Issue: 10, Pages: 2319 - 2327
Published: Jul 27, 2017
Abstract
The constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP ), which is one of the benchmark promoters of Pichia pastoris, was analyzed in terms of putative transcription factor binding sites. We constructed a synthetic library with distinct regulatory properties through deletion and duplication of these putative transcription factor binding sites and selected transcription factor (TF) genes were overexpressed or deleted to...
Paper Details
Title
Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production inPichia pastoris
Published Date
Jul 27, 2017
Volume
114
Issue
10
Pages
2319 - 2327
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