RF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates
Abstract
Amber codon suppression for the insertion of non-natural amino acids (nnAAs) is limited by competition with release factor 1 (RF1). Here we describe the genome engineering of a RF1 mutant strain that enhances suppression efficiency during cell-free protein synthesis, without significantly impacting cell growth during biomass production. Specifically, an out membrane protease (OmpT) cleavage site was engineered into the switch loop of RF1, which...
Paper Details
Title
RF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates
Published Date
Jun 8, 2017
Journal
Volume
7
Issue
1
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