A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica

Published on May 25, 2017in Insects
· DOI :10.3390/insects8020055
Michael E. Sparks6
Estimated H-index: 6
(USDA: United States Department of Agriculture),
Joshua H. Rhoades3
Estimated H-index: 3
(USDA: United States Department of Agriculture)
+ 6 AuthorsDawn E. Gundersen-Rindal16
Estimated H-index: 16
(USDA: United States Department of Agriculture)
The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected. Gene ontology and protein family analyses were conducted to explore the functional potential of the harlequin bug’s gene repertoire, and phylogenetic analyses were conducted on gene families germane to xenobiotic detoxification, including glutathione S-transferases, carboxylesterases and cytochrome P450s. Genic content in harlequin bug was compared with that of the closely related invasive pest, the brown marmorated stink bug, Halyomorpha halys (Stal). Quantitative analyses of harlequin bug gene expression levels, experimentally validated using quantitative real-time PCR, identified genes differentially expressed between life stages and/or sexes.
  • References (64)
  • Citations (5)
📖 Papers frequently viewed together
27 Citations
8 Authors (Fei Kou, ..., Wanzhi Cai)
1 Citations
47 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Peng He (Guida: Guizhou University)H-Index: 14
#2Yun-Fei ZhangH-Index: 1
Last. Ming He (Guida: Guizhou University)H-Index: 14
view all 9 authors...
Female moths synthesize species-specific sex pheromone components and release them to attract male moths, which depend on precise sex pheromone chemosensory system to locate females. Two types of genes involved in the sex pheromone biosynthesis and degradation pathways play essential roles in this important moth behavior. To understand the function of genes in the sex pheromone pathway, this study investigated the genome-wide and digital gene expression of sex pheromone biosynthesis and degradat...
13 CitationsSource
#1Robert L Koch (UMN: University of Minnesota)H-Index: 20
#2Daniela T. Pezzini (UMN: University of Minnesota)H-Index: 2
Last. Thomas E. Hunt (NU: University of Nebraska–Lincoln)H-Index: 17
view all 4 authors...
7 CitationsSource
#1Mallikarjuna Reddy Joga (UGent: Ghent University)H-Index: 2
#2Moisés João ZottiH-Index: 11
Last. Olivier Christiaens (UGent: Ghent University)H-Index: 18
view all 4 authors...
In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches towards insect pest management using RNAi requires first to unravel factors behind the efficiency of dsRNA-mediated gene silencing. In this review, we explore essential implications and possibilities to increase RNAi efficiency by delivery of ds...
101 CitationsSource
#1Anthony S. DiMeglio (VT: Virginia Tech)H-Index: 4
#2Anna K. Wallingford (Cornell University)H-Index: 10
Last. Donald E. Mullins (VT: Virginia Tech)H-Index: 16
view all 5 authors...
The harlequin bug, Murgantia histrionica (Hahn), is a serious pest of brassicaceous vegetables in southern North America. While this insect is limited in its northern range of North America, presumably by severe cold winter temperatures, specific information on its cold hardiness remains unknown. We determined the supercooling points (SCPs) for Maryland and Virginia adult populations and found no significant difference among these populations. SCPs were similar for adults ( X - = −10.35 °C; σ X ...
7 CitationsSource
#1Olivier Duron (CNRS: Centre national de la recherche scientifique)H-Index: 26
#2Valérie Noël (CNRS: Centre national de la recherche scientifique)H-Index: 9
Summary Pantoea are emerging as widespread bacteria engaged in associations with a variety of hosts, including plants, insects and humans. Recently, mutualistic Pantoea gut symbionts have also been reported in pentatomid stinkbugs. In the present study, we examine the incidence and evolutionary history of these Pantoea symbionts in pentatomid stinkbug populations from 14 species, characterising the processes that shape their diversity. We identify midgut crypts of pentatomid stinkbugs as harbori...
16 CitationsSource
#1Vasileia Balabanidou (FORTH: Foundation for Research & Technology – Hellas)H-Index: 4
#2Anastasia Kampouraki (UoC: University of Crete)H-Index: 4
Last. John Vontas (FORTH: Foundation for Research & Technology – Hellas)H-Index: 42
view all 15 authors...
The role of cuticle changes in insecticide resistance in the major malaria vector Anopheles gambiae was assessed. The rate of internalization of 14C deltamethrin was significantly slower in a resistant strain than in a susceptible strain. Topical application of an acetone insecticide formulation to circumvent lipid-based uptake barriers decreased the resistance ratio by ∼50%. Cuticle analysis by electron microscopy and characterization of lipid extracts indicated that resistant mosquitoes had a ...
72 CitationsSource
#1Zhitao Yu (Shanxi University)H-Index: 4
#2Xueyao Zhang (Shanxi University)H-Index: 9
Last. Jianzhen Zhang (Shanxi University)H-Index: 23
view all 8 authors...
Cytochrome P450 superfamily proteins play important roles in detoxification of xenobiotics and during physiological and developmental processes. To contribute to our understanding of this large gene family in insects, we have investigated the function of the cytochrome P450 gene LmCYP4G102 in the migratory locust Locusta migratoria. Suppression of LmCYP4G102 expression by RNA interference (RNAi) does not interfere with moulting but causes rapid loss of body weight - probably due to massive loss ...
20 CitationsSource
#1Franziska Beran (MPG: Max Planck Society)H-Index: 8
#2Peter Rahfeld (MPG: Max Planck Society)H-Index: 6
Last. Tobias G. Köllner (MPG: Max Planck Society)H-Index: 34
view all 11 authors...
Sesquiterpenes play important roles in insect communication, for example as pheromones. However, no sesquiterpene synthases, the enzymes involved in construction of the basic carbon skeleton, have been identified in insects to date. We investigated the biosynthesis of the sesquiterpene (6 R ,7 S )-himachala-9,11-diene in the crucifer flea beetle Phyllotreta striolata , a compound previously identified as a male-produced aggregation pheromone in several Phyllotreta species. A (6 R ,7 S )-himachal...
24 CitationsSource
Harlequin bug (Murgantia histrionica )i s an important pest of cole crops in the USA. The adults and nymphs feed on aboveground plant tissues by sucking cell contents and can seriously damage the host. Current insect control measures on cole crops target mainly lepidopteran pests, and the insecticides generally used do not control harlequin bug, so alternative management practices need to be explored. Previous research has established the existence of a male-produced pheromone attractive to both...
8 CitationsSource
#1Felipe A. Simão (Swiss Institute of Bioinformatics)H-Index: 9
#2Robert M. Waterhouse (Swiss Institute of Bioinformatics)H-Index: 35
Last. Evgeny M. Zdobnov (Swiss Institute of Bioinformatics)H-Index: 56
view all 5 authors...
Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal SingleCopy Orthologs, named BUSCO. A...
2,228 CitationsSource
Cited By5
#1Jeffrey L. Howell (UK: University of Kentucky)H-Index: 1
#2Kanakachari Mogilicherla (UK: University of Kentucky)H-Index: 6
Last. Subba Reddy Palli (UK: University of Kentucky)H-Index: 46
view all 4 authors...
#1Michael E. Sparks (ARS: Agricultural Research Service)H-Index: 4
#2Raman Bansal (ARS: Agricultural Research Service)
Last. Harsha Doddapaneni (BCM: Baylor College of Medicine)H-Index: 11
view all 57 authors...
BACKGROUND: Halyomorpha halys (Stal), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This species is also a nuisance due to overwintering in human-made structures. It has caused significant agricultural losses in recent years along the Atlantic seaboard of North America and in continental Europe. Genomic resources will assist with determining the molecular basis for this species' feeding and habitat traits, defining ...
#1Li‐Fang Li (Yunnan Agricultural University)H-Index: 1
#2Xi Gao (Yunnan Agricultural University)
Last. Guoxing Wu (Yunnan Agricultural University)H-Index: 3
view all 12 authors...
The fat body, a multifunctional organ analogous to the liver and fat tissue of vertebrates, plays an important role in insect life cycles. The fat body is involved in protein storage, energy metabolism, elimination of xenobiotics, and production of immunity regulator-like proteins. However, the molecular mechanism of the fat body’s physiological functions in the tephritid stem gall-forming fly, Procecidochares utilis, are still unknown. In this study, we performed transcriptome analysis of the f...
#1Lindsey C. Perkin (ARS: Agricultural Research Service)H-Index: 5
#2Brenda Oppert (ARS: Agricultural Research Service)H-Index: 34
#1Priyanka Mittapelly (OSU: Ohio State University)H-Index: 3
#2Raman Bansal (OSU: Ohio State University)H-Index: 5
Last. Andy Michel (OSU: Ohio State University)H-Index: 5
view all 3 authors...
#1Jason LancasterH-Index: 3
#2Bryan LehnerH-Index: 3
Last. Dorothea ThollH-Index: 28
view all 9 authors...
Insects use a wide range of structurally diverse pheromones for intra-specific communication. Compounds in the class of terpenes are emitted as sex, aggregation, alarm, or trail pheromones. Despite the common occurrence of terpene pheromones in different insect lineages, their origin from dietary host plant precursors or de novo biosynthetic pathways often remains unknown. Several stink bugs (Hemiptera: Pentatomidae) release bisabolene-type sesquiterpenes for aggregation and mating. Here we prov...
4 CitationsSource
#1Bernarda Calla (UIUC: University of Illinois at Urbana–Champaign)H-Index: 11
#2Marina MacLean (UNR: University of Nevada, Reno)H-Index: 5
Last. May R. Berenbaum (UIUC: University of Illinois at Urbana–Champaign)H-Index: 69
view all 7 authors...
6 CitationsSource
#1Jason Lancaster (VT: Virginia Tech)H-Index: 3
#2Ashot Khrimian (USDA: United States Department of Agriculture)H-Index: 18
Last. Dorothea Tholl (VT: Virginia Tech)H-Index: 28
view all 18 authors...
Insects use a diverse array of specialized terpene metabolites as pheromones in intraspecific interactions. In contrast to plants and microbes, which employ enzymes called terpene synthases (TPSs) to synthesize terpene metabolites, limited information from few species is available about the enzymatic mechanisms underlying terpene pheromone biosynthesis in insects. Several stink bugs (Hemiptera: Pentatomidae), among them severe agricultural pests, release 15-carbon sesquiterpenes with a bisabolen...
6 CitationsSource