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A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica

Published on May 25, 2017in Insects
· DOI :10.3390/insects8020055
Michael E. Sparks5
Estimated H-index: 5
(USDA: United States Department of Agriculture),
Joshua H. Rhoades1
Estimated H-index: 1
(USDA: United States Department of Agriculture)
+ 6 AuthorsDawn E. Gundersen-Rindal10
Estimated H-index: 10
(USDA: United States Department of Agriculture)
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Abstract
The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected. Gene ontology and protein family analyses were conducted to explore the functional potential of the harlequin bug’s gene repertoire, and phylogenetic analyses were conducted on gene families germane to xenobiotic detoxification, including glutathione S-transferases, carboxylesterases and cytochrome P450s. Genic content in harlequin bug was compared with that of the closely related invasive pest, the brown marmorated stink bug, Halyomorpha halys (Stal). Quantitative analyses of harlequin bug gene expression levels, experimentally validated using quantitative real-time PCR, identified genes differentially expressed between life stages and/or sexes.
  • References (64)
  • Citations (5)
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References64
Newest
Published on Dec 1, 2017in BMC Genomics3.50
Peng He7
Estimated H-index: 7
(Guida: Guizhou University),
Yun-Fei Zhang1
Estimated H-index: 1
+ 6 AuthorsMing He12
Estimated H-index: 12
(Guida: Guizhou University)
Female moths synthesize species-specific sex pheromone components and release them to attract male moths, which depend on precise sex pheromone chemosensory system to locate females. Two types of genes involved in the sex pheromone biosynthesis and degradation pathways play essential roles in this important moth behavior. To understand the function of genes in the sex pheromone pathway, this study investigated the genome-wide and digital gene expression of sex pheromone biosynthesis and degradat...
Published on Jan 1, 2017
Robert L. Koch20
Estimated H-index: 20
(UMN: University of Minnesota),
Daniela Tamara Pezzini2
Estimated H-index: 2
(UMN: University of Minnesota)
+ 1 AuthorsThomas E. Hunt16
Estimated H-index: 16
(NU: University of Nebraska–Lincoln)
Published on Nov 17, 2016in Frontiers in Physiology3.20
Mallikarjuna Reddy Joga2
Estimated H-index: 2
(UGent: Ghent University),
Moisés João Zotti9
Estimated H-index: 9
+ 1 AuthorsOlivier Christiaens16
Estimated H-index: 16
(UGent: Ghent University)
In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches towards insect pest management using RNAi requires first to unravel factors behind the efficiency of dsRNA-mediated gene silencing. In this review, we explore essential implications and possibilities to increase RNAi efficiency by delivery of ds...
Published on Oct 1, 2016in Environmental Microbiology Reports2.87
Olivier Duron25
Estimated H-index: 25
(CNRS: Centre national de la recherche scientifique),
Valérie Noël9
Estimated H-index: 9
(CNRS: Centre national de la recherche scientifique)
Summary Pantoea are emerging as widespread bacteria engaged in associations with a variety of hosts, including plants, insects and humans. Recently, mutualistic Pantoea gut symbionts have also been reported in pentatomid stinkbugs. In the present study, we examine the incidence and evolutionary history of these Pantoea symbionts in pentatomid stinkbug populations from 14 species, characterising the processes that shape their diversity. We identify midgut crypts of pentatomid stinkbugs as harbori...
Published on Oct 1, 2016in Environmental Entomology1.45
Anthony S. DiMeglio4
Estimated H-index: 4
(VT: Virginia Tech),
Anna Wallingford10
Estimated H-index: 10
(Cornell University)
+ 2 AuthorsDonald E. Mullins14
Estimated H-index: 14
(VT: Virginia Tech)
The harlequin bug, Murgantia histrionica (Hahn), is a serious pest of brassicaceous vegetables in southern North America. While this insect is limited in its northern range of North America, presumably by severe cold winter temperatures, specific information on its cold hardiness remains unknown. We determined the supercooling points (SCPs) for Maryland and Virginia adult populations and found no significant difference among these populations. SCPs were similar for adults ( X - = −10.35 °C; σ X ...
Vasileia Balabanidou4
Estimated H-index: 4
(FORTH: Foundation for Research & Technology – Hellas),
Anastasia Kampouraki4
Estimated H-index: 4
(UoC: University of Crete)
+ 12 AuthorsAmy Lynd11
Estimated H-index: 11
(LSTM: Liverpool School of Tropical Medicine)
The role of cuticle changes in insecticide resistance in the major malaria vector Anopheles gambiae was assessed. The rate of internalization of (14)C deltamethrin was significantly slower in a resistant strain than in a susceptible strain. Topical application of an acetone insecticide formulation to circumvent lipid-based uptake barriers decreased the resistance ratio by ∼50%. Cuticle analysis by electron microscopy and characterization of lipid extracts indicated that resistant mosquitoes had ...
Published on Jul 1, 2016in Scientific Reports4.01
Zhitao Yu4
Estimated H-index: 4
,
Xueyao Zhang8
Estimated H-index: 8
+ 5 AuthorsJianzhen Zhang21
Estimated H-index: 21
Citation: Yu, Z. T., Zhang, X. Y., Wang, Y. W., Moussian, B., Zhu, K. Y., Li, S., . . . Zhang, J. Z. (2016). LmCYP4G102: An oenocyte-specific cytochrome P450 gene required for cuticular waterproofing in the migratory locust, Locusta migratoria. Scientific Reports, 6, 11. doi:10.1038/srep29980
Franziska Beran8
Estimated H-index: 8
(MPG: Max Planck Society),
Peter Rahfeld6
Estimated H-index: 6
(MPG: Max Planck Society)
+ 8 AuthorsDavid G. Heckel52
Estimated H-index: 52
(MPG: Max Planck Society)
Sesquiterpenes play important roles in insect communication, for example as pheromones. However, no sesquiterpene synthases, the enzymes involved in construction of the basic carbon skeleton, have been identified in insects to date. We investigated the biosynthesis of the sesquiterpene (6 R ,7 S )-himachala-9,11-diene in the crucifer flea beetle Phyllotreta striolata , a compound previously identified as a male-produced aggregation pheromone in several Phyllotreta species. A (6 R ,7 S )-himachal...
Published on Mar 1, 2016in Journal of Pest Science5.13
Guillermo Cabrera Walsh4
Estimated H-index: 4
,
Anthony S. DiMeglio4
Estimated H-index: 4
+ 1 AuthorsDonald C. Weber26
Estimated H-index: 26
Harlequin bug (Murgantia histrionica )i s an important pest of cole crops in the USA. The adults and nymphs feed on aboveground plant tissues by sucking cell contents and can seriously damage the host. Current insect control measures on cole crops target mainly lepidopteran pests, and the insecticides generally used do not control harlequin bug, so alternative management practices need to be explored. Previous research has established the existence of a male-produced pheromone attractive to both...
Published on Oct 1, 2015in Bioinformatics4.53
Felipe A. Simão8
Estimated H-index: 8
(Swiss Institute of Bioinformatics),
Robert M. Waterhouse35
Estimated H-index: 35
(Swiss Institute of Bioinformatics)
+ 2 AuthorsEvgeny M. Zdobnov55
Estimated H-index: 55
(Swiss Institute of Bioinformatics)
Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal SingleCopy Orthologs, named BUSCO. A...
Cited By5
Newest
Published on May 23, 2019in PeerJ2.35
Lindsey Perkin5
Estimated H-index: 5
(ARS: Agricultural Research Service),
Brenda Oppert33
Estimated H-index: 33
(ARS: Agricultural Research Service)
Published on May 22, 2019in Journal of Economic Entomology1.78
Priyanka Mittapelly3
Estimated H-index: 3
(OSU: Ohio State University),
Raman Bansal12
Estimated H-index: 12
(OSU: Ohio State University),
Andy P. Michel5
Estimated H-index: 5
(OSU: Ohio State University)
Published on Feb 1, 2019in Journal of Chemical Ecology2.45
Jason Lancaster2
Estimated H-index: 2
,
Bryan Lehner2
Estimated H-index: 2
+ 6 AuthorsDorothea Tholl27
Estimated H-index: 27
Insects use a wide range of structurally diverse pheromones for intra-specific communication. Compounds in the class of terpenes are emitted as sex, aggregation, alarm, or trail pheromones. Despite the common occurrence of terpene pheromones in different insect lineages, their origin from dietary host plant precursors or de novo biosynthetic pathways often remains unknown. Several stink bugs (Hemiptera: Pentatomidae) release bisabolene-type sesquiterpenes for aggregation and mating. Here we prov...
Published on Oct 1, 2018in Insect Molecular Biology2.44
Bernarda Calla11
Estimated H-index: 11
(UIUC: University of Illinois at Urbana–Champaign),
Marina MacLean3
Estimated H-index: 3
(UNR: University of Nevada, Reno)
+ 4 AuthorsMay R. Berenbaum67
Estimated H-index: 67
(UIUC: University of Illinois at Urbana–Champaign)
Jason Lancaster2
Estimated H-index: 2
(VT: Virginia Tech),
Ashot Khrimian18
Estimated H-index: 18
(USDA: United States Department of Agriculture)
+ 15 AuthorsPaul E. Marek10
Estimated H-index: 10
(VT: Virginia Tech)
Insects use a diverse array of specialized terpene metabolites as pheromones in intraspecific interactions. In contrast to plants and microbes, which employ enzymes called terpene synthases (TPSs) to synthesize terpene metabolites, limited information from few species is available about the enzymatic mechanisms underlying terpene pheromone biosynthesis in insects. Several stink bugs (Hemiptera: Pentatomidae), among them severe agricultural pests, release 15-carbon sesquiterpenes with a bisabolen...
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