Changes in milt volume and sperm quality with time after an injection of recombinant Japanese eel luteinizing hormone in male Japanese eels

Fifteen males of the Japanese eel (9 as the Japanese eel recombinant LH (rLH) group and 6 as the 0.9% NaCl group), which had received 7 weekly injections of rLH (500 μg/kg BW/wk), were used in the experiment. Two days after the 7th injection, milt was collected until the males were spent just before an injection of rLH (500 μg/kg BW) or 0.9% NaCl solution. After the injection, the amount of expressible milt weight and its quality (percentage...