Match!

Crystallizing membrane proteins for structure determination: use of lipidic mesophases.

Published on Jun 1, 2009in Annual review of biophysics12.18
· DOI :10.1146/annurev.biophys.050708.133655
Martin Caffrey54
Estimated H-index: 54
Abstract
The principal route to determine the structure and the function and interactions of membrane proteins is via macromolecular crystallography. For macromolecular crystallography to be successful, structure-quality crystals of the target protein must be forthcoming, and crystallogenesis represents a major challenge. Several techniques are employed to crystallize membrane proteins, and the bulk of these techniques make direct use of solubilized protein-surfactant complexes by the more traditional, so-called in surfo methods. An alternative in meso approach, which employs a bicontinuous lipidic mesophase, has emerged as a method with considerable promise in part because it involves reconstitution of the solubilized protein back into a stabilizing and organizing lipid bilayer reservoir as a prelude to crystallogenesis. A hypothesis for how the method works at the molecular level and experimental evidence in support of the proposal are reviewed here. The latest advances, successes, and challenges associated with the method are described.
  • References (55)
  • Citations (158)
References55
Newest
#1Martin Caffrey (UL: University of Limerick)H-Index: 54
#2Vadim Cherezov (Scripps Research Institute)H-Index: 60
#1Veli-Pekka Jaakola (Scripps Research Institute)H-Index: 10
#2Mark T. Griffith (Scripps Research Institute)H-Index: 11
Last.Raymond C. Stevens (Scripps Research Institute)H-Index: 95
view all 8 authors...
#1Annemarie B. Wöhri (Chalmers University of Technology)H-Index: 8
#2Linda C. Johansson (University of Gothenburg)H-Index: 15
Last.Gillian Young (Glas.: University of Glasgow)H-Index: 2
view all 14 authors...
#1Michael A. Hanson (Scripps Research Institute)H-Index: 28
#2Vadim Cherezov (Scripps Research Institute)H-Index: 60
Last.Raymond C. Stevens (Scripps Research Institute)H-Index: 95
view all 9 authors...
#1Vadim Cherezov (Scripps Research Institute)H-Index: 60
#2Daniel M. Rosenbaum (Stanford University)H-Index: 22
Last.Brian K. Kobilka (Stanford University)H-Index: 109
view all 11 authors...
Cited By158
Newest
#1T. Kato (UTokyo: University of Tokyo)
#2Tomohiro Nishizawa (UTokyo: University of Tokyo)H-Index: 14
Last.Kunio HirataH-Index: 20
view all 6 authors...
#1Jackson E. Moore (Monash University, Clayton campus)H-Index: 2
#2Thomas M. McCoy (Monash University, Clayton campus)H-Index: 6
Last.Rico F. Tabor (Monash University, Clayton campus)H-Index: 14
view all 8 authors...
#1Laura M. Wingler (HHMI: Howard Hughes Medical Institute)H-Index: 6
#2Conor McMahon (Harvard University)H-Index: 6
Last.Andrew C. Kruse (Harvard University)H-Index: 29
view all 5 authors...
#1Matthieu Masureel (Stanford University)H-Index: 9
#2Yaozhong Zou (Stanford University)H-Index: 5
Last.David E. Shaw (Columbia University)H-Index: 63
view all 16 authors...
#1Lei Wang (University of Pittsburgh)H-Index: 1
#2Dandan Yao (CAS: Chinese Academy of Sciences)H-Index: 1
Last.Cheng Zhang (University of Pittsburgh)H-Index: 11
view all 9 authors...
#1H. Liu (University of Pittsburgh)H-Index: 1
#2H.R. Kim (SKKU: Sungkyunkwan University)H-Index: 1
Last.Cheng Zhang (University of Pittsburgh)H-Index: 11
view all 8 authors...
#1June Southall (Glas.: University of Glasgow)H-Index: 11
#2Sarah Henry (Glas.: University of Glasgow)H-Index: 4
Last.Richard J. Cogdell (Glas.: University of Glasgow)H-Index: 57
view all 9 authors...
View next paperCrystallizing Membrane Proteins Using Lipidic Mesophases