Plant regeneration from ploidy-stable cryopreserved embryogenic lines of the hybrid Pinus elliottii x P. caribaea

Clonal breeding programs of the highly important interspecific hybrid null Pinus elliottii var. elliottii null and null Pinus caribaea var. hondurensis null require the establishment of robust cryopreservation protocols, with no genetic variability. Embryonal masses of this hybrid were successfully cryopreserved by a fast slow-freezing method. The protocol was optimized by testing the effects of dimethyl sulfoxide (DMSO) based cryoprotectant...