In Vivo Biotinylation of Antigens in E. coli.
Abstract
Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used to immobilize an antigen during phage display selections of binders. Here we describe a method of in vivo biotinylation of proteins during expression in E. coli, by tagging the protein with the short biotin...
Paper Details
Title
In Vivo Biotinylation of Antigens in E. coli.
Published Date
Jan 1, 2017
Journal
Volume
1586
Pages
337 - 344
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