In Vivo Biotinylation of Antigens in E. coli.

Volume: 1586, Pages: 337 - 344
Published: Jan 1, 2017
Abstract
Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used to immobilize an antigen during phage display selections of binders. Here we describe a method of in vivo biotinylation of proteins during expression in E. coli, by tagging the protein with the short biotin...
Paper Details
Title
In Vivo Biotinylation of Antigens in E. coli.
Published Date
Jan 1, 2017
Volume
1586
Pages
337 - 344
Citation AnalysisPro
  • Scinapse’s Top 10 Citation Journals & Affiliations graph reveals the quality and authenticity of citations received by a paper.
  • Discover whether citations have been inflated due to self-citations, or if citations include institutional bias.