Marker-free coselection for CRISPR-driven genome editing in human cells
Abstract
Targeted genome editing enables the creation of bona fide cellular models for biological research and may be applied to human cell-based therapies. Therefore, broadly applicable and versatile methods for increasing its efficacy in cell populations are highly desirable. We designed a simple and robust coselection strategy for enrichment of cells with either nuclease-driven nonhomologous end joining (NHEJ) or homology-directed repair (HDR) events...
Paper Details
Title
Marker-free coselection for CRISPR-driven genome editing in human cells
Published Date
Jun 1, 2017
Journal
Volume
14
Issue
6
Pages
615 - 620
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