Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions

Published on Sep 26, 2017in Philosophical Transactions of the Royal Society B6.139
· DOI :10.1098/rstb.2016.0384
Steven M. Driever11
Estimated H-index: 11
(University of Essex),
Andrew J. Simkin24
Estimated H-index: 24
(University of Essex)
+ 7 AuthorsChristine A. Raines40
Estimated H-index: 40
(University of Essex)
To meet the growing demand for food, substantial improvements in yields are needed. This is particularly the case for wheat, where global yield has stagnated in recent years. Increasing photosynthesis has been identified as a primary target to achieve yield improvements. To increase leaf photosynthesis in wheat, the level of the Calvin–Benson cycle enzyme sedoheptulose-1,7-biphosphatase (SBPase) has been increased through transformation and expression of a Brachypodium distachyon SBPase gene construct. Transgenic lines with increased SBPase protein levels and activity were grown under greenhouse conditions and showed enhanced leaf photosynthesis and increased total biomass and dry seed yield. This showed the potential of improving yield potential by increasing leaf photosynthesis in a crop species such as wheat. The results are discussed with regard to future strategies for further improvement of photosynthesis in wheat.
Figures & Tables
  • References (48)
  • Citations (42)
📖 Papers frequently viewed together
262 Citations
36 Citations
7 Authors (J. Kromdijk, ..., Stephen P. Long)
270 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Andrew J. Simkin (University of Essex)H-Index: 24
#2Patricia E. Lopez-Calcagno (University of Essex)H-Index: 7
Last. Christine A. Raines (University of Essex)H-Index: 40
view all 8 authors...
36 CitationsSource
#1Fei DingH-Index: 5
#2Meiling WangH-Index: 6
Last. Xizhen AiH-Index: 1
view all 4 authors...
Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants
19 CitationsSource
#1Andrew J. Simkin (University of Essex)H-Index: 24
#2Lorna McAusland (University of Essex)H-Index: 7
Last. Christine A. Raines (University of Essex)H-Index: 40
view all 5 authors...
72 CitationsSource
#1Caroline A. Sparks (Rothamsted Research)H-Index: 18
#2Huw Jones (Rothamsted Research)H-Index: 37
Since its first invention in the late 1980s the particle gun has evolved from a basic gunpowder driven machine firing tungsten particles to one more refined which uses helium gas as the propellant to launch alternative heavy metal particles such as gold and silver. The simple principle is that DNA-coated microscopic particles (microcarriers) are accelerated at high speed by helium gas within a vacuum and travel at such a velocity as to penetrate target cells. However, the process itself involves...
14 CitationsSource
126k Citations
#1Deepak K. Ray (UMN: University of Minnesota)H-Index: 21
#2Nathaniel D. Mueller (UMN: University of Minnesota)H-Index: 25
Last. Jonathan A. Foley (UMN: University of Minnesota)H-Index: 78
view all 4 authors...
Several studies have shown that global crop production needs to double by 2050 to meet the projected demands from rising population, diet shifts, and increasing biofuels consumption. Boosting crop yields to meet these rising demands, rather than clearing more land for agriculture has been highlighted as a preferred solution to meet this goal. However, we first need to understand how crop yields are changing globally, and whether we are on track to double production by 2050. Using ∼2.5 million ag...
840 CitationsSource
#1Martin A. J. Parry (Rothamsted Research)H-Index: 46
#2P. John Andralojc (Rothamsted Research)H-Index: 17
Last. Spencer M. Whitney (ANU: Australian National University)H-Index: 34
view all 7 authors...
Rubisco (ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase) enables net carbon fixation through the carboxylation of RuBP. However, some characteristics of Rubisco make it surprisingly inefficient and compromise photosynthetic productivity. For example, Rubisco catalyses a wasteful reaction with oxygen that leads to the release of previously fixed CO2 and NH3 and the consumption of energy during photorespiration. Furthermore, Rubisco is slow and large amounts are needed to support adequate ...
192 CitationsSource
#1Matthew P. Reynolds (CIMMYT: International Maize and Wheat Improvement Center)H-Index: 60
#2John Foulkes (University of Nottingham)H-Index: 13
Last. Gustavo A. Slafer (Catalan Institution for Research and Advanced Studies)H-Index: 59
view all 8 authors...
Wheat provides 20% of calories and protein consumed by humans. Recent genetic gains are <1% per annum (p.a.), insufficient to meet future demand. The Wheat Yield Consortium brings expertise in photosynthesis, crop adaptation and genetics to a common breeding platform. Theory suggest radiation use efficiency (RUE) of wheat could be increased ∼50%; strategies include modifying specificity, catalytic rate and regulation of Rubisco, up-regulating Calvin cycle enzymes, introducing chloroplast CO2 con...
215 CitationsSource
#1David TilmanH-Index: 142
#2Christian Balzer (UCSB: University of California, Santa Barbara)H-Index: 3
Last. Belinda L. Befort (UMN: University of Minnesota)H-Index: 1
view all 4 authors...
Global food demand is increasing rapidly, as are the environmental impacts of agricultural expansion. Here, we project global demand for crop production in 2050 and evaluate the environmental impacts of alternative ways that this demand might be met. We find that per capita demand for crops, when measured as caloric or protein content of all crops combined, has been a similarly increasing function of per capita real income since 1960. This relationship forecasts a 100–110% increase in global cro...
2,248 CitationsSource
#1David M. Rosenthal (USDA: United States Department of Agriculture)H-Index: 21
#2Anna M. Locke (UIUC: University of Illinois at Urbana–Champaign)H-Index: 8
Last. Donald Richard Ort (USDA: United States Department of Agriculture)H-Index: 61
view all 6 authors...
Background: Biochemical models predict that photosynthesis in C3 plants is most frequently limited by the slower of two processes, the maximum capacity of the enzyme Rubisco to carboxylate RuBP (Vc,max), or the regeneration of RuBP via electron transport (J). At current atmospheric [CO2] levels Rubisco is not saturated; consequently, elevating [CO2] increases the velocity of carboxylation and inhibits the competing oxygenation reaction which is also catalyzed by Rubisco. In the future, leaf phot...
92 CitationsSource
Cited By42
#2Marjorie Reyes-Díaz (UFRO: University of La Frontera)H-Index: 16
view all 8 authors...
Abstract Titanium dioxide nanoparticles (TiO2NPs) generally have positive effects on growth and physiological parameters in plants, but their impacts on photosynthesis and antioxidant system are poorly understood. This work aimed to assess the effects of TiO2 NPs on photosynthetic performance and antioxidant system in Raphanus sativus L. plants. Treatments consisted of TiO2 NPs and microparticles (μPs) applied foliarly by increasing doses from 10 to 1500 mg L−1, using deionized water as a contro...
#1Heng ZhangH-Index: 2
Last. Xiue WangH-Index: 18
view all 9 authors...
#1Goetz Hensel (Leibniz Association)H-Index: 19
Abstract Triticeae cereals are among the most important crop plants grown worldwide and being used for animal feed, food and beverages. Although breeding efforts evolved over the last ten thousand years our today's crop plants, biotechnological methods would help to speed up the process and incorporate traits impossible by conventional breeding. The main research topics were related to cover the future demand on our agricultural practices to supply sufficient food for a growing world population....
#1Robert T. Furbank (ANU: Australian National University)H-Index: 59
#2Robert E. Sharwood (ANU: Australian National University)H-Index: 19
Last. Anthony G. Condon (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 33
view all 5 authors...
Photosynthesis has become a major trait of interest for cereal yield improvement as breeders appear to have reached the theoretical genetic limit for harvest index, the mass of grain as a proportion of crop biomass. Yield improvements afforded by the adoption of green revolution dwarfing genes to wheat and rice are becoming exhausted and improvements in biomass and radiation use efficiency are now sought in these crops. Exploring genetic diversity in photosynthesis is now possible using high thr...
3 CitationsSource
#1Matthew J. Paul (Rothamsted Research)H-Index: 39
#2Amy Watson (Rothamsted Research)H-Index: 1
Last. Cara A. Griffiths (Rothamsted Research)H-Index: 11
view all 3 authors...
Understanding processes in sources and sinks that contribute to crop yields has taken years of painstaking research. For crop yield improvement, processes need to be understood as standalone mechanisms in addition to how these mechanisms perform at the crop level; currently there is often a chasm between the two. Fundamental mechanisms need to be considered in the context of crop ideotypes and the agricultural environment which is often more water than carbon limited. Different approaches for im...
1 CitationsSource
#1Ajay Kohli (IRRI: International Rice Research Institute)H-Index: 24
#1Jacqueline d'A Hughes (Rice University)
Last. Jacqueline d’A Hughes (IRRI: International Rice Research Institute)H-Index: 1
view all 4 authors...
Despite impressive success in molecular physiological understanding of photosynthesis, and preliminary evidence on its potential for quantum shifts in agricultural productivity, the question remains if increased photosynthesis, without parallel fine-tuning of the associated processes is enough. There is a distinct lack of formal socio-economic impact studies that address the critical questions on product profiling, cost-benefit analysis, environmental trade-offs, and technological and market for...
1 CitationsSource
#1Xia LiH-Index: 36
#2Peng Wang (CAS: Chinese Academy of Sciences)H-Index: 55
Last. Wenbin Zhou (CAAS: Civil Aviation Authority of Singapore)
view all 9 authors...
Photosynthetic efficiency is a major target for improvement of crop yield potential under agricultural field conditions. Inefficiencies can occur in many steps of the photosynthetic process, from chloroplast biogenesis to functioning of the light harvesting and carbon fixation reactions. Nuclear-encoded GOLDEN2-LIKE (GLK) transcription factors regulate some of the earliest steps by activating target genes encoding chloroplast-localized and photosynthesis-related proteins. Here we show that const...
Last. Wagner L. AraújoH-Index: 34
view all 6 authors...
Abstract Much attention has been given to the enhancement of photosynthesis as a strategy for the optimization of crop productivity. As traditional plant breeding is most likely reaching a plateau, there is a timely need to accelerate improvements in photosynthetic efficiency by means of novel tools and biotechnological solutions. The emerging field of synthetic biology offers the potential for building completely novel pathways in predictable directions and, thus, addresses the global requireme...
1 CitationsSource
#1Rut Sanchez-Bragado (University of Lleida)H-Index: 6
#2Rubén Vicente (MPG: Max Planck Society)H-Index: 8
Last. José Luis Araus (University of Barcelona)H-Index: 51
view all 6 authors...
Small grain cereals such as wheat, rice and barley are among the most important crops worldwide. Any attempt to increase crop productivity and stability through breeding implies developing new strategies for plant phenotyping, including defining ideotype attributes for selection. Recently, the role of non-foliar photosynthetic organs, particularly the inflorescences, has received increasing attention. For example, ear photosynthesis has been reported to be a major contributor to grain filling in...
1 CitationsSource