EGFR gene regulation in colorectal cancer cells by garlic phytocompounds with special emphasis on S-Allyl-L-Cysteine Sulfoxide

Published on Dec 1, 2018in Interdisciplinary Sciences: Computational Life Sciences1.418
· DOI :10.1007/s12539-017-0227-6
Nabarun Roy3
Estimated H-index: 3
(Kerala Agricultural University),
P. A. Nazeem5
Estimated H-index: 5
(Kerala Agricultural University)
+ 5 AuthorsAchuthan C. Raghavamenon5
Estimated H-index: 5
(University of Calicut)
Colorectal cancer is one among the most common cancers in the world and a major cause of cancer related deaths. Similar to other cancers, colorectal carcinogenesis is often associated with over expression of genes related to cell growth and proliferation, especially Epidermal Growth Factor Receptor (EGFR). There is an increasing attention towards the plant derived compounds in prevention of colorectal carcinogenesis by downregulating EGFR. Among plants, garlic (Allium sativum L.) is emerging with anticancer properties by virtue of its organosulfur compounds. The present study was aimed to analyze the interaction ability of garlic compounds in the active region of EGFR gene by in silico molecular docking studies and in vitro validation. This was conducted using the Discovery studio software version 4.0. Among the tested compounds, s-allyl-l-cysteine-sulfoxide (SACS)/alliin showed higher affinity towards EGFR. Furthermore, wet lab analysis using cell viability test and EGFR expression analysis in colorectal cancer cells confirmed its efficacy as a potent anticancer agent.
  • References (59)
  • Citations (4)
📖 Papers frequently viewed together
10 Authors (Gwang Hun Park, ..., Jin Boo Jeong)
1 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Jie Zheng (SYSU: Sun Yat-sen University)H-Index: 10
#2Yue Zhou (SYSU: Sun Yat-sen University)H-Index: 14
Last. Hua-Bin Li (SYSU: Sun Yat-sen University)H-Index: 25
view all 6 authors...
Spices have been widely used as food flavorings and folk medicines for thousands of years. Numerous studies have documented the antioxidant, anti-inflammatory and immunomodulatory effects of spices, which might be related to prevention and treatment of several cancers, including lung, liver, breast, stomach, colorectum, cervix, and prostate cancers. Several spices are potential sources for prevention and treatment of cancers, such as Curcuma longa (tumeric), Nigella sativa (black cumin), Zingibe...
54 CitationsSource
13 Citations
#1Gudrun A. Fridgeirsdottir (University of Nottingham)H-Index: 2
#2Robert J. Harris (University of Nottingham)H-Index: 2
Last. Clive J. Roberts (University of Nottingham)H-Index: 54
view all 4 authors...
The need for solubility enhancement through formulation is a well-known but still problematic issue because of the numbers of poorly water-soluble drugs in development. There are several possible routes that can be taken to increase the bioavailability of drugs intended for immediate-release oral formulation. The best formulation strategy for any given drug will depend on numerous factors, including required dose, shelf life, manufacturability, and the properties of the active pharmaceutical ing...
10 CitationsSource
Breast cancer (BC) is a serious lifestyle disease. There are several prognostic biomarkers like nuclear receptors whose over-expression is associated with BC characteristics. These biomarkers can be blocked by compounds with anti-cancer potential but selection must be made on the basis of no adverse side effects. This study is focused on finding of compounds from a plant source garlic.Twenty compounds from garlic and five targets considered involved in BC were retrieved from Pubchem database and...
3 Citations
#1Yongqiang ChenH-Index: 14
#2Elizabeth S. Henson (UM: University of Manitoba)H-Index: 15
Last. Spencer B. Gibson (UM: University of Manitoba)H-Index: 42
view all 7 authors...
ABSTRACTAutophagy is an intracellular lysosomal degradation pathway where its primary function is to allow cells to survive under stressful conditions. Autophagy is, however, a double-edge sword that can either promote cell survival or cell death. In cancer, hypoxic regions contribute to poor prognosis due to the ability of cancer cells to adapt to hypoxia in part through autophagy. In contrast, autophagy could contribute to hypoxia induced cell death in cancer cells. In this study, we showed th...
33 CitationsSource
#1Jerome de Ruyck (university of lille)H-Index: 3
Last. Marc F. LensinkH-Index: 21
view all 4 authors...
New molecular modeling approaches, driven by rapidly improving computational platforms, have allowed many success stories for the use of computer-assisted drug design in the discovery of new mechanism-or structure-based drugs. In this overview, we highlight three aspects of the use of molecular docking. First, we discuss the combination of molecular and quantum mechanics to investigate an unusual enzymatic mechanism of a flavoprotein. Second, we present recent advances in anti-infectious agents’...
38 CitationsSource
#1Kaiming Wang (SDU: Shandong University)H-Index: 5
#2Ying Wang (SDU: Shandong University)H-Index: 3
Last. Shanzhong LiH-Index: 5
view all 12 authors...
Abstract The anti -cancer effects of oil-soluble organosulfur compounds in garlic in the initiation phase of carcinogenesis are known. However, there are few experimental studies investigating S -allylmercaptocysteine (SAMC), a water-soluble derivative of garlic. This study investigated whether SAMC prevented the carcinogen benzo(a)pyrene (B(a)P) from inducing precancerous activity in human lung cells (A549 cell line). A549 cells were either pre-treated (PreTM) or concurrently treated (CoTM) wit...
6 CitationsSource
#1Arwa Bin Raies (KAUST: King Abdullah University of Science and Technology)H-Index: 4
#2Vladimir B. Bajic (KAUST: King Abdullah University of Science and Technology)H-Index: 48
Determining the toxicity of chemicals is necessary to identify their harmful effects on humans, animals, plants, or the environment. It is also one of the main steps in drug design. Animal models have been used for a long time for toxicity testing. However, in vivo animal tests are constrained by time, ethical considerations, and financial burden. Therefore, computational methods for estimating the toxicity of chemicals are considered useful. In silico toxicology is one type of toxicity assessme...
114 CitationsSource
Chemoprevention is one of the cancer prevention approaches wherein natural/synthetic agent(s) are prescribed with the aim to delay or disrupt multiple pathways and processes involved at multiple steps, i.e., initiation, promotion, and progression of cancer. Amongst environmental chemopreventive compounds, diet/beverage-derived components are under evaluation, because of their long history of exposure to humans, high tolerability, low toxicity, and reported biological activities. This compilation...
36 CitationsSource
#1Manuela Chiavarini (University of Perugia)H-Index: 7
#2Liliana Minelli (University of Perugia)H-Index: 16
Last. Roberto Fabiani (University of Perugia)H-Index: 20
view all 3 authors...
Objective Colorectal cancer shows large incidence variations worldwide that have been attributed to different dietary factors. We conducted a meta-analysis on the relationship between garlic consumption and colorectal cancer risk. Design We systematically reviewed publications obtained by searching ISI Web of Knowledge, MEDLINE and EMBASE literature databases. We extracted the risk estimate of the highest and the lowest reported categories of intake from each study and conducted meta-analysis us...
13 CitationsSource
Cited By4
#1M. OsorioH-Index: 6
Last. Cristina CastroH-Index: 15
view all 4 authors...
Colorectal cancer (CRC) is the type with the second highest morbidity. Recently, a great number of bioactive compounds and encapsulation techniques have been developed. Thus, this paper aims to review the drug delivery strategies for chemotherapy adjuvant treatments for CRC, including an initial scientific-technological analysis of the papers and patents related to cancer, CRC, and adjuvant treatments. For 2018, a total of 167,366 cancer-related papers and 306,240 patents were found. Adjuvant tr...
#2Anthony J. OveryH-Index: 1
Last. Dietrich BüsselbergH-Index: 31
view all 3 authors...
Gastrointestinal (GI) cancer is a prevailing global health disease with a high incidence rate which varies by region. It is a huge economic burden on health care providers. GI cancer affects different organs in the body such as the gastric organs, colon, esophagus, intestine, and pancreas. Internal and external factors like smoking, obesity, urbanization, genetic mutations, and prevalence of Helicobacter pylori and Hepatitis B and Hepatitis C viral infections could increase the risk of GI cancer...
1 CitationsSource
#1Arunaksharan Narayanankutty (St. Joseph's College, Bangalore)H-Index: 1
#2Joice Tom Job (St. Joseph's College, Bangalore)H-Index: 1
Last. Vinayak Narayanankutty (Government Medical College, Thiruvananthapuram)H-Index: 1
view all 3 authors...
2 CitationsSource
#1Vinayak Narayanankutty (Government Medical College, Thiruvananthapuram)H-Index: 1
#2Arunaksharan Narayanankutty (St. Joseph's College, Devagiri)H-Index: 1
Last. Anusree NairH-Index: 1
view all 3 authors...
1 CitationsSource
#1Dl Sheela (Kerala Agricultural University)H-Index: 1
#2Arunaksharan Narayanankutty (University of Calicut)H-Index: 7
Last. Sr Muthangaparambil (Kerala Agricultural University)H-Index: 1
view all 5 authors...
Coconut oil (CO) is enriched with medium chain saturated fatty acids like lauric acid (LA), capric acid and caprylic acid, which are known to have several health benefits. LA, the predominant fatty...
1 CitationsSource
#1Rui-Feng ZengH-Index: 2
#2Jin-Shuai LanH-Index: 6
Last. Yue DingH-Index: 10
view all 8 authors...
A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection l...
5 CitationsSource