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EGFR gene regulation in colorectal cancer cells by garlic phytocompounds with special emphasis on S-Allyl-L-Cysteine Sulfoxide

Published on Dec 1, 2018in Interdisciplinary Sciences: Computational Life Sciences1.42
· DOI :10.1007/s12539-017-0227-6
Nabarun Roy2
Estimated H-index: 2
(Kerala Agricultural University),
P. Nazeem4
Estimated H-index: 4
(Kerala Agricultural University)
+ 5 AuthorsAchuthan C. Raghavamenon5
Estimated H-index: 5
(University of Calicut)
Cite
Abstract
Colorectal cancer is one among the most common cancers in the world and a major cause of cancer related deaths. Similar to other cancers, colorectal carcinogenesis is often associated with over expression of genes related to cell growth and proliferation, especially Epidermal Growth Factor Receptor (EGFR). There is an increasing attention towards the plant derived compounds in prevention of colorectal carcinogenesis by downregulating EGFR. Among plants, garlic (Allium sativum L.) is emerging with anticancer properties by virtue of its organosulfur compounds. The present study was aimed to analyze the interaction ability of garlic compounds in the active region of EGFR gene by in silico molecular docking studies and in vitro validation. This was conducted using the Discovery studio software version 4.0. Among the tested compounds, s-allyl-l-cysteine-sulfoxide (SACS)/alliin showed higher affinity towards EGFR. Furthermore, wet lab analysis using cell viability test and EGFR expression analysis in colorectal cancer cells confirmed its efficacy as a potent anticancer agent.
  • References (59)
  • Citations (2)
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References59
Newest
Published on Aug 12, 2016in Nutrients4.17
Jie Zheng9
Estimated H-index: 9
(SYSU: Sun Yat-sen University),
Yue Zhou14
Estimated H-index: 14
(SYSU: Sun Yat-sen University)
+ 3 AuthorsHua-Bin Li42
Estimated H-index: 42
(SYSU: Sun Yat-sen University)
Spices have been widely used as food flavorings and folk medicines for thousands of years. Numerous studies have documented the antioxidant, anti-inflammatory and immunomodulatory effects of spices, which might be related to prevention and treatment of several cancers, including lung, liver, breast, stomach, colorectum, cervix, and prostate cancers. Several spices are potential sources for prevention and treatment of cancers, such as Curcuma longa (tumeric), Nigella sativa (black cumin), Zingibe...
Published on Aug 1, 2016in Journal of Pharmaceutical Sciences3.20
Gudrun A. Fridgeirsdottir1
Estimated H-index: 1
(University of Nottingham),
Robert J. Harris1
Estimated H-index: 1
(University of Nottingham)
+ 1 AuthorsClive J. Roberts51
Estimated H-index: 51
(University of Nottingham)
The need for solubility enhancement through formulation is a well-known but still problematic issue because of the numbers of poorly water-soluble drugs in development. There are several possible routes that can be taken to increase the bioavailability of drugs intended for immediate-release oral formulation. The best formulation strategy for any given drug will depend on numerous factors, including required dose, shelf life, manufacturability, and the properties of the active pharmaceutical ing...
Nabarun Roy2
Estimated H-index: 2
,
Sangeetha P. Davis1
Estimated H-index: 1
+ 5 AuthorsAchuthan C. Raghavamenon5
Estimated H-index: 5
Breast cancer (BC) is a serious lifestyle disease. There are several prognostic biomarkers like nuclear receptors whose over-expression is associated with BC characteristics. These biomarkers can be blocked by compounds with anti-cancer potential but selection must be made on the basis of no adverse side effects. This study is focused on finding of compounds from a plant source garlic.Twenty compounds from garlic and five targets considered involved in BC were retrieved from Pubchem database and...
Published on Jun 2, 2016in Autophagy11.06
Yongqiang Chen13
Estimated H-index: 13
,
Elizabeth S. Henson14
Estimated H-index: 14
(UM: University of Manitoba)
+ 4 AuthorsSpencer B. Gibson42
Estimated H-index: 42
(UM: University of Manitoba)
ABSTRACTAutophagy is an intracellular lysosomal degradation pathway where its primary function is to allow cells to survive under stressful conditions. Autophagy is, however, a double-edge sword that can either promote cell survival or cell death. In cancer, hypoxic regions contribute to poor prognosis due to the ability of cancer cells to adapt to hypoxia in part through autophagy. In contrast, autophagy could contribute to hypoxia induced cell death in cancer cells. In this study, we showed th...
Published on Jun 1, 2016
Jérôme de Ruyck3
Estimated H-index: 3
(university of lille),
Guillaume Brysbaert9
Estimated H-index: 9
+ 1 AuthorsMarc F. Lensink20
Estimated H-index: 20
New molecular modeling approaches, driven by rapidly improving computational platforms, have allowed many success stories for the use of computer-assisted drug design in the discovery of new mechanism-or structure-based drugs. In this overview, we highlight three aspects of the use of molecular docking. First, we discuss the combination of molecular and quantum mechanics to investigate an unusual enzymatic mechanism of a flavoprotein. Second, we present recent advances in anti-infectious agents’...
Published on May 1, 2016in International Immunopharmacology3.36
Kaiming Wang5
Estimated H-index: 5
(SDU: Shandong University),
Ying Wang3
Estimated H-index: 3
(SDU: Shandong University)
+ 9 AuthorsJianhua Cai5
Estimated H-index: 5
Abstract The anti -cancer effects of oil-soluble organosulfur compounds in garlic in the initiation phase of carcinogenesis are known. However, there are few experimental studies investigating S -allylmercaptocysteine (SAMC), a water-soluble derivative of garlic. This study investigated whether SAMC prevented the carcinogen benzo(a)pyrene (B(a)P) from inducing precancerous activity in human lung cells (A549 cell line). A549 cells were either pre-treated (PreTM) or concurrently treated (CoTM) wit...
Arwa Bin Raies3
Estimated H-index: 3
(KAUST: King Abdullah University of Science and Technology),
Vladimir B. Bajic46
Estimated H-index: 46
(KAUST: King Abdullah University of Science and Technology)
Determining the toxicity of chemicals is necessary to identify their harmful effects on humans, animals, plants, or the environment. It is also one of the main steps in drug design. Animal models have been used for a long time for toxicity testing. However, in vivo animal tests are constrained by time, ethical considerations, and financial burden. Therefore, computational methods for estimating the toxicity of chemicals are considered useful. In silico toxicology is one type of toxicity assessme...
Published on Feb 1, 2016in Public Health Nutrition2.53
Manuela Chiavarini6
Estimated H-index: 6
(University of Perugia),
Liliana Minelli16
Estimated H-index: 16
(University of Perugia),
Roberto Fabiani20
Estimated H-index: 20
(University of Perugia)
Objective Colorectal cancer shows large incidence variations worldwide that have been attributed to different dietary factors. We conducted a meta-analysis on the relationship between garlic consumption and colorectal cancer risk. Design We systematically reviewed publications obtained by searching ISI Web of Knowledge, MEDLINE and EMBASE literature databases. We extracted the risk estimate of the highest and the lowest reported categories of intake from each study and conducted meta-analysis us...
Published on Feb 1, 2016in Journal of Nutrition4.42
Craig S Charron2
Estimated H-index: 2
(ARS: Agricultural Research Service),
Harry Dawson26
Estimated H-index: 26
(ARS: Agricultural Research Service),
Janet A. Novotny24
Estimated H-index: 24
(ARS: Agricultural Research Service)
There is a large body of preclinical research aimed at understanding the roles of garlic and garlic-derived preparations in the promotion of human health. Most of this research has targeted the possible functions of garlic in maintaining cardiovascular health and in preventing and treating cancer. A wide range of outcome variables has been used to investigate the bioactivity of garlic, ranging from direct measures of health status such as cholesterol concentrations, blood pressure, and changes i...
Cited By2
Newest
Published on May 9, 2019in Current Drug Targets2.64
Vinayak Narayanankutty1
Estimated H-index: 1
(Government Medical College, Thiruvananthapuram),
Arunaksharan Narayanankutty1
Estimated H-index: 1
(St. Joseph's College, Devagiri),
Anusree Nair1
Estimated H-index: 1
Published on Apr 3, 2019in Human & Experimental Toxicology2.17
Dl Sheela (Kerala Agricultural University), Arunaksharan Narayanankutty6
Estimated H-index: 6
(University of Calicut)
+ 2 AuthorsSr Muthangaparambil (Kerala Agricultural University)
Published on Sep 26, 2017in Molecules3.06
Rui-Feng Zeng2
Estimated H-index: 2
,
Jin-Shuai Lan4
Estimated H-index: 4
+ 5 AuthorsYue Ding9
Estimated H-index: 9
A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection l...
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