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Physicochemical properties and cellular protection against oxidation of degraded Konjac glucomannan prepared by γ-irradiation

Published on Sep 1, 2017in Food Chemistry 5.40
· DOI :10.1016/j.foodchem.2017.03.121
Wenjie Jian4
Estimated H-index: 4
(SCAU: South China Agricultural University),
Liying Tu1
Estimated H-index: 1
(JMU: Jimei University)
+ 3 AuthorsYuanming Sun20
Estimated H-index: 20
(SCAU: South China Agricultural University)
Cite
Abstract
Abstract Konjac glucomannan (KGM) is an important functional polysaccharide in food research. However, unstable dispersibility of KGM inhibits its in-depth study and wide application. In this study, a degraded KGM (100 kGy-KGM), which showed excellent dispersibility and specific physicochemical properties, were obtained by γ-irradiation in a dosage of 100 kGy. We investigated the protective effect of 100 kGy-KGM against H 2 O 2 induced oxidative damage in LO2 cells. Our results demonstrated that pretreatment of LO2 cells with 100 kGy-KGM not only significantly increased cellular survivals and activities of GSH-Px and CAT, but also reduced levels of LDH, MDA and intracellular accumulation of ROS. The marked protective effect against oxidative damage and excellent dispersibility in 100 kGy-KGM allowed its possible use as an antioxidant. Our study provided fundamental knowledge to understand the structure-functions relationships of degraded-KGM, which could result in a theoretical guidance for the future application of KGM.
  • References (39)
  • Citations (8)
Cite
References39
Newest
Published on Oct 1, 2016in Carbohydrate Polymers 6.04
Wenjie Jian4
Estimated H-index: 4
(SCAU: South China Agricultural University),
Huayi Wu1
Estimated H-index: 1
+ 4 AuthorsYuanming Sun20
Estimated H-index: 20
(SCAU: South China Agricultural University)
Abstract Konjac glucomannan (KGM) is an important gelling agent in composite gels. This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP). In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10 kGy-KGM, 20 kGy-KGM, and 100 kGy-KGM) of different molecular characteristics. Native KGM, 10 kGy-KGM, and 20 kGy-KGM exerted negative effect...
Published on Jun 1, 2016in Experimental and Toxicologic Pathology
Sudha Govindan1
Estimated H-index: 1
(Science College),
Elizabeth Elcy Rani Johnson1
Estimated H-index: 1
(Science College)
+ 3 AuthorsJayanthi Gopalan2
Estimated H-index: 2
(Government Arts College, Coimbatore)
Abstract The crude polysaccharides were extracted from the fruiting bodies of Calocybe indica (CIP). The antioxidant activities of CIP were evaluated both in vitro and in vivo . Chemical characteristics of the polysaccharides were investigated. In in vitro antioxidant assay, CIP showed noticeable 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radical scavenging activities, reducing power and lipid peroxidation inhibition. Chemical analysis showed the presence of carbohydrate, protein and the FT...
Published on Feb 1, 2016in Carbohydrate Polymers 6.04
Ka-Chai Siu8
Estimated H-index: 8
(PolyU: Hong Kong Polytechnic University),
Lijian Xu4
Estimated H-index: 4
(Heilongjiang University)
+ 1 AuthorsJian-Yong Wu44
Estimated H-index: 44
(PolyU: Hong Kong Polytechnic University)
Abstract This study aims to discover novel and bioactive polysaccharides (PS) from wild Armillaria ostoyae , a honey mushroom species. Two PS designated AkPS1V-1 (66.6 kDa) and AkPS1V-2 (15.3 kDa) were isolated and fractionated by anion ion exchange (IEC) and size exclusion chromatography (SEC) from the alkaline extract of A. ostoyae mushrooms. AkPS1V-1 was a glucan composed of solely glucose residues and AkPS1V-2 a heteropolysaccharide composed of glucose and galactose at 6:1 molar ratio. AkPS1...
Published on Dec 1, 2015in Carbohydrate Polymers 6.04
Wenjie Jian2
Estimated H-index: 2
(PolyU: Hong Kong Polytechnic University),
Ka-Chai Siu8
Estimated H-index: 8
(PolyU: Hong Kong Polytechnic University),
Jian-Yong Wu44
Estimated H-index: 44
(PolyU: Hong Kong Polytechnic University)
Abstract The hydrocolloidal characteristics of Konjac glucomannan (KGM) are important for its application as a thickening and gelling agent for liquid foods. In this study, the rheological behavior and molecular properties such as molar mass, hydrodynamic radius and chain conformation of KGM in water were determined at various pH levels (4.0–10.0) during heating from 20 to 80 °C. Acidic and neutral conditions (pH 4.0–7.0) promoted the dispersion of KGM, and alkaline condition at pH 10 favored it...
Published on Oct 1, 2015in Carbohydrate Polymers 6.04
Jianhua Liu4
Estimated H-index: 4
(Zhejiang University of Technology),
Qiuhong Xu4
Estimated H-index: 4
(Zhejiang University of Technology)
+ 4 AuthorsYuting Ding6
Estimated H-index: 6
(Zhejiang University of Technology)
Abstract Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using β-mannanase. The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates. Factors affecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions were as follows: time 2 h; temperature 50 °C; pH 6.0; and enzymatic concentration 150 U/g. Under these optimized conditions, minimum viscosity (31.9 mPa·s) of the hydrolasate was obtained. The av...
Published on Jan 1, 2015in Food Chemistry 5.40
Yalda Shokoohinia13
Estimated H-index: 13
(Kermanshah University of Medical Sciences),
Maryam Rashidi2
Estimated H-index: 2
(Kermanshah University of Medical Sciences)
+ 1 AuthorsZohreh Jelodarian2
Estimated H-index: 2
(Kermanshah University of Medical Sciences)
Abstract Since flavonols are antioxidant agents, they could in principle, beneficially affect neurodegenerative diseases where reactive oxygen species are involved. Quercetin derivatives are the most abundant dietary flavonoids, and we have investigated the capacity of quercetin-3- O - β - d -glucopyranoside (Q3G) isolated from Echinophora cinerea to protect PC12 cells from H 2 O 2 -induced cytotoxicity. Direct cytotoxic effects of H 2 O 2 on PC12 in presence and absence of Q3G were evaluated. H...
Published on Jan 1, 2015in Neurotoxicity Research 3.31
Carolina Echeverry10
Estimated H-index: 10
,
Florencia Arredondo10
Estimated H-index: 10
+ 3 AuthorsFederico Dajas20
Estimated H-index: 20
Few studies have been undertaken on the relationship of the structure of flavones and neuroprotection. Previously, we described the structural determinants of the neuroprotective activity of some natural flavones in cerebellar granule neurons in culture against an oxidative insult (H2O2). In the present work, we analyzed anti-oxidant activity, cellular iron, and Ca2+ levels and cellular bioavailability of neuroprotective and nonneuroprotective flavones in the same experimental paradigm. Oxidativ...
Published on May 1, 2014in Food Hydrocolloids 5.84
Weiping Jin13
Estimated H-index: 13
,
Wei Xu14
Estimated H-index: 14
+ 4 AuthorsBin Li21
Estimated H-index: 21
(MOE: Chinese Ministry of Education)
Abstract 60 Co γ-ray irradiation assisted with ethanol pretreatment was utilized to prepare degraded konjac glucomannan products with well-dispersed low molecular fractions and white color. The molecular weight decreased from 1.4 × 10 6 to 6 × 10 5 at the dose of 3.6 kGy irradiation with 50% ethanol pretreatment. The properties and structure were analyzed by Fourier-transform infrared spectroscopy (FT-IR), colorimeter, dynamic light scattering (DLS), differential scanning calorimetry (DSC) and r...
Published on Apr 1, 2014in Drug and Chemical Toxicology 1.95
Gaurav Kaithwas10
Estimated H-index: 10
(BBAU: Babasaheb Bhimrao Ambedkar University),
Prashant Singh1
Estimated H-index: 1
(BBAU: Babasaheb Bhimrao Ambedkar University),
Daksh Bhatia2
Estimated H-index: 2
AbstractIn the present study, the antioxidant activity of the polysaccharides from aloe vera (Aloe barbadensis Miller) gel was evaluated, in vitro by five established methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH–) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide scavenging, superoxide radical (O-2) scavenging and reducing power assay, and in vivo against doxorubicin (DOX)-induced myocardial oxidative stress (OS) in albino wistar rats. The polysaccharides exhibited significant ...
Published on Mar 1, 2014in Free Radical Research 2.83
M. Ramalingam1
Estimated H-index: 1
(Kyung Hee University),
S.-J. Kim1
Estimated H-index: 1
(Kyung Hee University)
AbstractOxidative stress is induced by excess accumulation of reactive oxygen and nitrogen species (RONS). Astrocytes are metabolically active cells in the brain and understanding astrocytic responses to oxidative stress is essential to understand brain pathologies. In addition to direct oxidative stress, exogenous hydrogen peroxide (H2O2) can penetrate biological membranes and enhance formation of other RONS. The present study was carried out to examine the role of insulin in H2O2-induced oxida...
Cited By8
Newest
Published on Jul 1, 2019in International Journal of Biological Macromolecules 4.78
Lin Wang5
Estimated H-index: 5
,
Ruo-Jun Mu5
Estimated H-index: 5
+ 4 AuthorsJie Pang9
Estimated H-index: 9
Abstract In this study, a facile marine bioinspired surface modification approach for carboxyl-functionalized multiwalled carbon nanotube (CCNT) and enhanced interfacial adhesion with the konjac glucomannan (KGM) matrix were illustrated to develop aerogels. Combined with FT-IR, XRD, Raman, TGA, XPS and SEM results, it was indicated that functionalized CCNT (PCCNT) is a reinforcer through hydrogen bond interactions in the aerogel formation process, which could be the main reason for the enhanceme...
Published on May 1, 2019in International Journal of Biological Macromolecules 4.78
Wanmei Lin1
Estimated H-index: 1
(Fujian Agriculture and Forestry University),
Yongsheng Ni3
Estimated H-index: 3
(Fujian Agriculture and Forestry University)
+ 3 AuthorsJie Pang9
Estimated H-index: 9
(Fujian Agriculture and Forestry University)
Abstract The exploration of methods for degrading konjac glucomannan (KGM) is of great significant and technological interest. Here, laser at the power of 10 W was employed to degrade KGM. The laser degraded konjac glucomannan (LDK) was analyzed by viscosity, Rheology, Differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy and laser light scatter (LLS). The viscosity of the LDK decreased from 8.38 Pa·s to 2.26 Pa·s and the average molecular weight (Mw) decreased...
Lin Wang5
Estimated H-index: 5
(Fujian Agriculture and Forestry University),
Lizhuan Lin (Fujian Agriculture and Forestry University)+ 2 AuthorsJie Pang9
Estimated H-index: 9
(Fujian Agriculture and Forestry University)
Abstract To development of konjac glucomannan (KGM) based films for use in food packaging is increasing because of environmental attraction, biodegradability and availability. The objective of this research was to prepare a facile approach for KGM based films. Polydopamine (PDA) functionalized microcrystalline cellulose (PFMC) were incorporated into a KGM matrix. The KGM/PFMC films were prepared by the solvent casting method. The surface morphology of the KGM/PFMC films were generated by scannin...
Published on Jan 1, 2019in Cyta-journal of Food 1.60
Qiaoran Zheng (Yangtze Normal University), Wenfeng Li (Yangtze Normal University)+ 4 AuthorsYan Zhang (Yangtze Normal University)
ABSTRACTThe ultrasound-degraded oxidized konjac glucomannan (U-OKGM) was manufactured and its immune activity in RAW264.7 macrophages was examined. Results showed that ultrasound treatment, which was an effective method to obtain low molecular weight OKGM, did not change the main structure of polysaccharides under the test conditions. Molecular weights (MW) of OKGM and U-OKGM are about 1.58 × 106 g/mol and 5.64 × 105 g/mol, respectively. We showed that U-OKGM could inhibit NO secretion and augme...
Published on Dec 1, 2018in Carbohydrate Polymers 6.04
Honglei Chen3
Estimated H-index: 3
(SWU: Southwest University),
Junwen Cheng1
Estimated H-index: 1
+ 5 AuthorsFei Lu5
Estimated H-index: 5
(SWU: Southwest University)
Abstract Hydrogels with self-healing capacity can undergo self-repair, establishing safer and longer-lasting products. Hydrogel wound dressings showing self-healing capacity can prolong the lifespan of the material and provide better wound protection. Therefore, in this study, Schiff base reactions (reversible imine linkages) were utilized to design injectable self-healing hydrogels with chitosan and konjac glucomannan. Oxidized konjac glucomannan was used to react with chitosan to form hydrogel...
Published on Aug 1, 2018in Food Chemistry 5.40
Fan Zhu14
Estimated H-index: 14
(University of Auckland)
Abstract Konjac glucomannan (KGM) is a major polysaccharide from the corm of Amorphophallus konjac. Native KGM has limited uses and has been chemically/physically/enzymatically modified to expand the range of functional properties. This mini-review summarises the recent advances of modifying KGM for diverse food and nonfood applications, focusing on the chemical and physical modifications. The chemical methods include substitution, grafting, cross-linking, oxidation, and deacetylation, whereas t...
Published on May 1, 2018in Food Research International 3.58
Wenjie Jian4
Estimated H-index: 4
(SCAU: South China Agricultural University),
Yung-Husan Chen + 3 AuthorsYuanming Sun20
Estimated H-index: 20
(SCAU: South China Agricultural University)
Abstract Konjac glucomannan (KGM) is an important source for preparation of Konjac oligo-glucomannan (KOG), but high molecular weight and viscosity in KGM inhibited its full degradation into KOG. In this study, KOG with a degree of polymerization between 2 and 9 was obtained by combining γ-irradiation and enzymatic hydrolysis in high yield. We investigated the protective effect of KOG against H 2 O 2 - induced oxidative damage in vitro, using human hepatic cell line (LO2) as a cell model. Our re...
Published on Jan 1, 2018in Food Research International 3.58
Ester Hernández-Corroto1
Estimated H-index: 1
(University of Alcalá),
María Luisa Marina31
Estimated H-index: 31
(University of Alcalá),
María Concepción García13
Estimated H-index: 13
(University of Alcalá)
Abstract The long exposition to reactive species results in oxidative stress which has been related with the development of cancer and other serious diseases. Olea europaea and Prunus persica seeds present a high protein content and preliminary results demonstrated their high potency to obtain bioactive peptides. The protective effect against oxidative damage exerted by peptides released from Olea europaea and Prunus persica seeds has been evaluated in this work. Seed hydrolysates showed protect...