Biomineralisation with Saos-2 bone cells on TiSiN sputtered Ti alloys

Published on Jul 1, 2017in Colloids and Surfaces B: Biointerfaces3.973
· DOI :10.1016/j.colsurfb.2017.03.039
V. V. Anusha Thampi3
Estimated H-index: 3
(CSIR: Council of Scientific and Industrial Research),
Avi Bendavid33
Estimated H-index: 33
(CSIRO: Commonwealth Scientific and Industrial Research Organisation)
+ 4 AuthorsBalasubramanian Subramanian23
Estimated H-index: 23
(CSIR: Council of Scientific and Industrial Research)
Abstract Surface modifications of metallic implants are important in order to protect the underlying metals from the harsh corrosive environment inside the human body and to minimize the losses caused by wear. Recently, researches are carried out in developing bioactive surfaces on metallic implants, which supports the growth and proliferation of cells on to these surfaces. Titanium silicon nitride (TiSiN) hard nanocomposites thin films were fabricated on Ti alloys (Ti-6Al–4 V) by pulsed direct current (DC) reactive magnetron sputtering. The films were characterized for its microstructural and electrochemical behavior. The higher charge transfer resistance (Rct) and positive shift in Ecorr value of TiSiN/Ti alloys than the bare Ti-alloys indicates a better corrosion resistance offered by the TiSiN thin films to the underlying substrates. The biological response to TiSiN/Ti alloys and control bare Ti-alloys was measured in vitro using cell-based assays with two main outcomes. Firstly, neither the Ti alloy nor the TiSiN thin film was cytotoxic to cells. Secondly, the TiSiN thin film promoted differentiation of human bone cells above the bare control Ti alloy as measured by alkaline phosphatase and calcium production. TiSiN thin films provide better corrosion resistance and protect the underlying metal from the corrosive environment. The thin film surface is both biocompatible and bioactive as indicated from the cytotoxicity and biomineralization studies.
  • References (21)
  • Citations (2)
📖 Papers frequently viewed together
6 Citations
10 Citations
6 Authors (Shidong Zhang, ..., Hongtao Li)
1 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1P. M. Perillo (National Atomic Energy Commission)H-Index: 2
The present study deals with growth of TiN coatings deposited by plasma enhanced physical vapor deposition (PEPVD) on commercial titanium substrate and zircaloy-4. The composition, microstructure and residual stresses of the coatings were determined by scanning electron microscopy (SEM), photoelectron spectroscopy generated by X-rays (XPS/ ESCA) and X-ray diffraction. The anodic behaviour of TiN layers were investigated by means of potentiodynamic anodic polarization curves and galvanostatic exp...
3 Citations
#1Cancan Zhao (CAS: Chinese Academy of Sciences)H-Index: 4
#2Lunguo Xia (SJTU: Shanghai Jiao Tong University)H-Index: 26
Last. Kaili Lin (CAS: Chinese Academy of Sciences)H-Index: 39
view all 8 authors...
Patterned structured surfaces are very useful to control a cell's microenvironment and to modulate certain cellular responses, such as cell adhesion, migration, proliferation, and differentiation. Herein, ordered micropatterns constructed by a quadrate convex with different sizes were fabricated on a hydroxyapatite [Ca10(PO4)6(OH)2, HAp] bioceramic surface using an ordered micropatterned nylon sieve as templates. The height, width and space of the convex for the patterns could be facilely regula...
30 CitationsSource
Objectives: To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application. Study Design: Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscop...
12 CitationsSource
#1M. Arab Pour Yazdi (UTBM: Universite de technologie de Belfort-Montbeliard)H-Index: 8
#2F. Lomello (UTBM: Universite de technologie de Belfort-Montbeliard)H-Index: 3
Last. Alain Billard (UTBM: Universite de technologie de Belfort-Montbeliard)H-Index: 16
view all 9 authors...
Abstract TiSiN nanocomposites coatings were synthesized for the first time by a hybrid deposition technique where high power impulse (HiPIMS) and pulsed-DC (PDCMS) magnetron co-sputtering were used for Ti and Si deposition respectively in an Ar + N2 atmosphere. For the Ti target, the deposition parameters were fixed, while the current applied to the Si target ranged from 0 to 0.9 A. Thus, the Si content in the films was adjusted from 0 to 8.8 at.% Si to allows tailoring of microstructure and mec...
20 CitationsSource
#1P. Trivedi (IITR: Indian Institute of Technology Roorkee)H-Index: 9
#2Pushpendra K. Gupta (IITR: Indian Institute of Technology Roorkee)H-Index: 37
Last. Partha Pratim Roy (IITR: Indian Institute of Technology Roorkee)H-Index: 30
view all 6 authors...
Abstract Amongst the Ti alloys used as orthopedic implant materials, Ti6Al4V is one of the widely used alloys. Magnetron sputtering was used to deposit nanocomposite coating of Ti–Si–N on the Ti6Al4V substrate at different power and then the coating structure and surface properties were characterized through contact angle measurement, X-ray diffraction (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). In vitro biocompatibility of the coatings was assessed by using mou...
21 CitationsSource
#1P. Jojibabu (Indian Institute of Technology Madras)H-Index: 1
#2B. Ratna Sunil (Indian Institute of Technology Madras)H-Index: 7
Last. Mukesh Doble (Indian Institute of Technology Madras)H-Index: 42
view all 6 authors...
Commercial purity titanium (CP-Ti) was processed by equal channel angular pressing (ECAP) with a channel angle of 120° with the aim of studying the effect of microstructure on the wettability and bioactivity. Solid and tubular Ti rods of 6 mm diameter were ECAPed at room temperature. During ECAP of tubular specimens, hydroxyapatite was filled in the tube. Grains and sub-grains of size approximately 1 μm were observed using transmission electron microscopy in both solid and tubular samples. Vicke...
7 CitationsSource
#1Ingolf EndlerH-Index: 5
#2Mandy HöhnH-Index: 4
Last. Martin Knaut (TUD: Dresden University of Technology)H-Index: 10
view all 6 authors...
Abstract TiN and TiC x N y are commercial CVD coatings widely used for cutting tools. A promising route for improving their performance is the addition of silicon. Therefore the main objective of this work is the preparation of TiSiN and TiSiCN coatings with a nanocomposite structure offering a higher hardness as well as an improved oxidation and wear resistance. New low pressure CVD processes in the range 800 °C–900 °C were developed for the successful deposition of nanocomposite coatings of Ti...
22 CitationsSource
#1Hsi-Kai Tsou (FCU: Feng Chia University)H-Index: 16
#2Ping-Yen Hsieh (FCU: Feng Chia University)H-Index: 7
Last. Ju-Liang He (FCU: Feng Chia University)H-Index: 23
view all 5 authors...
Titanium dioxide (TiO2), known to exhibit good biocompatibility, is applied in this study as a thin film formed onto polyetheretherketone (PEEK) substrate, which has been widely used in spinal interbody fusion cages. For successful deposition, an arc ionplating (AIP) technique was applied to deposit TiO2 at low deposition temperature without damaging PEEK substrate, while providing satisfactory film adhesion. This study systematically investigates the effects of TiO2 thin film phase composition ...
21 CitationsSource
#1Ling LiH-Index: 4
#2Kyle CrosbyH-Index: 4
Last. Yong WangH-Index: 21
view all 5 authors...
Hydroxyapatite (HA) is the main inorganic component of human hard tissue. It is bioactive and supports bone in growth and osteointegration, and thus it is widely used for orthopedic, dental and maxillofacial applications. To fully utilize its potential in forming a strong bone-implant interface, we have investigated the effect of surface roughness of HA on cell attachment and cell proliferation. The HA samples are prepared via sintering, followed by polishing and then scratch generation using a ...
11 CitationsSource
#1Subramanian Balasubramanian (CSIR: Council of Scientific and Industrial Research)H-Index: 3
#2Ananthakumar Ramadoss (CSIR: Council of Scientific and Industrial Research)H-Index: 19
Last. Jayachandran Muthirulandi (CSIR: Council of Scientific and Industrial Research)H-Index: 2
view all 4 authors...
Nanocomposite Ti–Si–N coatings were prepared by reactive dc magnetron sputtering in a mixture of Ar and N2 gases onto bio implantable 316L stainless steel substrates. X-ray diffraction analysis revealed that the Ti–Si–N nanocomposite coatings are mainly composed of amorphous Si3N4 and TiN crystals. The presence of different phases like TiN, TiO2, and Si3N4 was confirmed from X-ray photoelectron spectroscopy analysis. Raman spectra of the as-deposited composite coatings exhibited characteristic p...
12 CitationsSource
Cited By2
#1D.G. Liu (Hefei University of Technology)H-Index: 13
#2Yan LiangH-Index: 2
Last. Li. Zhang (Hefei University of Technology)H-Index: 1
view all 6 authors...
Abstract Ti+C/amorphous carbon (a-C) gradient multilayer (GM) films are prepared on the Ti-alloy substrates via physical vapor deposition. Transmission electron microscopy revealed that the Ti atoms combine with the a-C film to form a TiC phase in the inner layer and the sputtering current significantly influences the amount of the TiC phase. Further, the mechanical properties of the Ti+C/a-C GM films were obtained using nanoindentation, and the results denoted the significant improvement in the...
#1S. Thanka Rajan (AcSIR: Academy of Scientific and Innovative Research)H-Index: 1
#2Avi Bendavid (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 33
Last. B. Subramanian (AcSIR: Academy of Scientific and Innovative Research)H-Index: 3
view all 3 authors...
Abstract Biologically safe Ti-based quaternary Ti-Nb-Zr-Si thin film metallic glass (TFMG) was fabricated by sputtering on Titanium alloy (Ti6Al4V or Ti alloy) substrates. A preliminary assessment regarding glass forming ability, thermal stability and corrosion behavior was performed. The amorphous nature of the film is evidenced from the X-ray Diffraction (XRD) and Transmission Electron Microscope (TEM) and Selected Area Electron Diffraction (SAED) patterns. Ion scattering spectroscopy (ISS) an...
4 CitationsSource