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  • References (98)
  • Citations (7)
Alexander McPherson53
Estimated H-index: 53
(UCI: University of California, Irvine),
Yurii G. Kuznetsov16
Estimated H-index: 16
(UCI: University of California, Irvine)
The nucleation and growth of protein, nucleic acid and virus crystals from solution are functions of underlying kinetic and thermodynamic parameters that govern the process, and these are all supersaturation-dependent. While the mechanisms of macromolecular crystal growth are essentially the same as for conventional crystals, the underlying parameters are vastly different, in some cases orders of magnitude lower, and this produces very different crystallization processes. Numerous physical featu...
Published on Jan 1, 2012in Physics Procedia
Hitoshi Wada20
Estimated H-index: 20
(National Institute for Materials Science),
Noriyuki Hirota15
Estimated H-index: 15
(National Institute for Materials Science)
+ 10 AuthorsAkiko Kita16
Estimated H-index: 16
(Kyoto University)
Abstract A quasi-microgravity environment appears in a high-field superconducting magnet bore where a large magnetic force counterbalances gravity acting on a diamagnetic substance. This suppresses convection of the diamagnetic solution in the crystallization cell placed in the bore from which protein crystals precipitate. A 16 T class superconducting magnet has been developed with a special coil configuration; one of the component coils produces a magnetic field the direction of which is opposi...
Christopher A. Kors2
Estimated H-index: 2
(Argonne National Laboratory),
Ellen Wallace5
Estimated H-index: 5
+ 3 AuthorsPeter Nollert21
Estimated H-index: 21
When starting a protein-crystallization project, scientists are faced with several unknowns. Amongst them are these questions: (i) is the purity of the starting material sufficient? and (ii) which type of crystallization experiment is the most promising to conduct? The difficulty in purifying active membrane-protein samples for crystallization trials and the high costs associated with producing such samples require an extremely pragmatic approach. Additionally, practical guidelines are needed to...
Published on Dec 3, 2008in Crystal Growth & Design 4.15
Luis Antonio González-Ramírez8
Estimated H-index: 8
Jordi Carrera1
Estimated H-index: 1
+ 2 AuthorsJuan Manuel García-Ruiz32
Estimated H-index: 32
6 pages, 3 figures.-- Supporting information (Video animation of the FOTON M-3 Mission and Photographs showing two views of the GCF) available at: http://pubs.acs.org/doi/suppl/10.1021/cg800788e
Published on Apr 1, 2006in Journal of Crystal Growth 1.57
Izumi Yoshizaki14
Estimated H-index: 14
(JAXA: Japan Aerospace Exploration Agency),
Seijiro Fukuyama5
Estimated H-index: 5
+ 10 AuthorsLong Rong5
Estimated H-index: 5
(JAXA: Japan Aerospace Exploration Agency)
Abstract The rocking curve widths of tetragonal lysozyme crystals were measured. Typical full-width half-maximum (FWHM) of the rocking curve of crystals grown from a pure lysozyme solution were 0.004–0.006°, while those crystals containing 5% lysozyme dimer were 0.006–0.010°. X-ray topography experiments revealed that dislocations existed in crystals containing the lysozyme dimer. These dislocations are one of the causes of rocking curve width broadening. 2×10 4 cm −2 pits were observed on the {...
Published on Apr 1, 2005in Reports on Progress in Physics 16.62
Edward H. Snell23
Estimated H-index: 23
(MSFC: Marshall Space Flight Center),
John R. Helliwell36
Estimated H-index: 36
(University of Manchester)
Density difference fluid flows and sedimentation of growing crystals are greatly reduced when crystallization takes place in a reduced gravity environment. In the case of macromolecular crystallography a crystal of a biological macromolecule is used for diffraction experiments (x-ray or neutron) so as to determine the three-dimensional structure of the macromolecule. The better the internal order of the crystal then the greater the molecular structure detail that can be extracted. It is this str...
Published on Apr 1, 2003in Journal of Structural Biology 3.75
Joseph D. Ng13
Estimated H-index: 13
(UAH: University of Alabama in Huntsville),
Jose A. Gavira23
Estimated H-index: 23
(UAH: University of Alabama in Huntsville),
Juan Manuel García-Ruiz32
Estimated H-index: 32
(CSIC: Spanish National Research Council)
Counterdiffusion crystallization in capillary is a very simple, cost-effective, and practical procedure for obtaining protein crystals suitable for X-ray data analysis. Its principles have been derived using well-known concepts coupling the ideas of precipitation and diffusion mass transport in a restricted geometry. The counterdiffusion process has been used to simultaneously screen for optimal conditions for protein crystal growth, incorporate strong anomalous scattering atoms, and mix in cryo...
Juan Ma. García-Ruiz8
Estimated H-index: 8
Luis Antonio González-Ramírez8
Estimated H-index: 8
+ 1 AuthorsFermín Otálora22
Estimated H-index: 22
Granada Crystallisation Box (GCB) is a new crystallisation device designed to perform counter-diffusion experiments. Here we describe the device and its use for protein crystallisation purposes. GCB allows one to explore and exploit the coupling between crystallisation and diffusion. The role of viscous fluids, gels and/or microgravity can be enhanced by using capillary volumes, creating a perfect diffusive mass transport scenario. The use of capillaries also reduces the consumption of macromole...
Published on Jan 1, 2001in Crystal Growth & Design 4.15
Craig E. Kundrot3
Estimated H-index: 3
Russell A. Judge13
Estimated H-index: 13
+ 2 AuthorsM. Franklin Rose1
Estimated H-index: 1
Macromolecular crystal growth is seen as an ideal experiment to make use of the reduced acceleration environment provided by an orbiting spacecraft. The experiments are small, are simply operated, and have a high potential scientific and economic impact. In this review we examine the theoretical reasons why microgravity is a beneficial environment for crystal growth and survey the history of experiments on the Space Shuttle Orbiter, on unmanned spacecraft, and on the Mir space station. The resul...
Published on Aug 15, 1999in Proteins
Craig L. Caylor8
Estimated H-index: 8
(Cornell University),
Ivan Dobrianov7
Estimated H-index: 7
(Cornell University)
+ 8 AuthorsA.A. Chernov1
Estimated H-index: 1
(UAH: University of Alabama in Huntsville)
The mechanisms by which macromolecular impurities degrade the diffraction properties of protein crystals have been investigated using X-ray topography, high-resolution diffraction line shape measurements, crystallographic data collection, chemical analysis, and two-photon excitation fluorescence microscopy. Hen egg-white lysozyme crystals grown from solutions containing a structurally unrelated protein (ovotransferrin) and a related protein (turkey egg-white lysozyme) can exhibit significantly b...
Cited By7
Troy J. Scott2
Estimated H-index: 2
(RTP: Research Triangle Park),
Nicholas S. Vonortas25
Estimated H-index: 25
(GW: George Washington University)
A basic mission of NASA is to use the United States’ segment of the International Space Station (ISS), designated a national laboratory, to facilitate the growth of a commercial marketplace in low Earth orbit for scientific research, technology development, observation and communications. Protein crystallization research has long been promoted as a promising commercial application of the ISS for drug development. In this paper we examine the case for microgravity protein crystallization under di...
Published on Jan 1, 2019
Masatoshi Maeki10
Estimated H-index: 10
(Hokkaido University),
Manabu Tokeshi38
Estimated H-index: 38
(Hokkaido University)
Protein crystallization and its three-dimensional structure analysis is indispensable for understanding the protein function in the body and life phenomenon. Three dimensional structure of protein also plays important role for drug discovery and it have been already used to design new drug. To determine the three dimensional protein structure, protein crystallization conditions: concentration of protein, kinds and concentration of precipitant, buffer, pH, temperature, and additives must be optim...
Published on Feb 4, 2018in bioRxiv
Sebastian Mattea (Goethe University Frankfurt), Marco A. S. Baptista7
Estimated H-index: 7
(Michael J. Fox Foundation)
+ 5 AuthorsStefan Knapp76
Estimated H-index: 76
(Goethe University Frankfurt)
Mutations in the gene coding for leucine-rich repeat kinase 2 (LRRK2) are a considerable cause for Parkinson9s disease (PD). However, the high-resolution 3D structure of the protein is still lacking. This structure will not only help to understand PD etiology but will also enable rational drug design. We have established a reliable method to produce LRRK2 crystals for the first time. However, the limited resolution of the diffraction data prevented structure determination using crystallographic ...
Published on Dec 1, 2017
Die Forschungsfreiheit ist in den meisten Staaten im Grundrecht verbrieft. Im Weltraum begegnen wir jedoch einer Reihe von Schwierigkeiten, wenn es darum geht, diese Freiheit in Anspruch zu nehmen. Sei dies bedingt durch die uns zur Verfugung stehende unangepasste Technik, unseren limitierten physiologischen und kognitiven Fahigkeiten, fehlendem Zugang oder mangelnder finanzieller Mittel. Der Weltraum ist deshalb eine Terra incognita geblieben, allerdings mit einem unbestrittenen Innovationspote...
Published on Jul 6, 2017
Evgeniya Rubin1
Estimated H-index: 1
Christopher Owen2
Estimated H-index: 2
Vivian Stojanoff18
Estimated H-index: 18
Electric fields have been employed to promote macromolecular crystallization for several decades. Although crystals grown in electric fields seem to present higher diffraction quality, these methods are not widespread. For most configurations, electrodes are in direct contact with the protein solution. Here, we propose a configuration that can be easily extended to standard crystallization methods for which the electrodes are not in direct contact with the protein solution. Furthermore, the prop...
Published on Jan 1, 2017
Günter Ruyters1
Estimated H-index: 1
(DLR: German Aerospace Center),
Christian Betzel38
Estimated H-index: 38
(UHH: University of Hamburg)
The utilization of microgravity for improving protein crystallization and thereby structure determination started in the early 1980s onboard of TEXUS sounding rockets and of the US Space Shuttle. After the successful pioneering work by Prof. Littke, especially the German space life sciences program put much effort into this topic. In spite of some technical and methodological drawbacks, early successes could be obtained as well. In some cases, microgravity experiments enabled crystallization of ...
Published on Aug 1, 2016in EMBO Reports 8.38
Andrea C. Rinaldi26
Estimated H-index: 26
Ground Control to Major Tom Ground Control to Major Tom Take your protein pills and put your helmet on Ground Control to Major Tom (Ten, Nine, Eight, Seven, Six) Commencing countdown, engines on (Five, Four, Three) Check ignition and may God's love be with you (Two, One, Liftoff) —David Bowie: Space Oddity Humans have been going into space for a number of reasons: to “beat the other side” during the Cold War, out of curiosity, to make the first tentative steps into the great beyond or simply “be...
Published on Jul 20, 2016in Bioconjugate Chemistry 4.35
Giarita Ferraro10
Estimated H-index: 10
Chiara Gabbiani35
Estimated H-index: 35
Antonello Merlino29
Estimated H-index: 29
The X-ray structure of the adduct formed in the reaction between the gold N-heterocyclic carbene compound Au(NHC)Cl (with NHC = 1-butyl-3-methyl-imidazole-2-ylidene) and the model protein thaumatin is reported here. The structure reveals binding of Au(NHC)+ fragments to distinct protein sites. Notably, binding of the gold compound occurs at lysine side chains and at the N-terminal tail; the metal binds the protein after releasing Cl– ligand, but retaining NHC fragment.