A Modular Toolkit for Generating Pichia pastoris Secretion Libraries
Abstract
Yeasts are powerful eukaryotic hosts for the production of recombinant proteins due to their rapid growth to high cell densities and ease of genetic modification. For large-scale industrial production, secretion of a protein offers the advantage of simple and efficient downstream purification that avoids costly cell rupture, denaturation and refolding. The methylotrophic yeast Pichia pastoris (Komagataella phaffi) is a well-established...
Paper Details
Title
A Modular Toolkit for Generating Pichia pastoris Secretion Libraries
Published Date
Mar 15, 2017
Journal
Volume
6
Issue
6
Pages
1016 - 1025
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