Bioactivity studies of Huh-7 cells derived human epidermal growth factor expressed in Pichia pastoris

Volume: 81, Issue: 6, Pages: 1114 - 1119
Published: Mar 9, 2017
Abstract
Previously, we have reported cloning of human epidermal growth factor gene from Huh-7 cells and its extracellular expression in Pichia pastoris. The presented work is a detailed report regarding molecular characterization of Huh-7 cells-derived hEGF expressed in Pichia pastoris with special reference to its glycosylation profiling and bioactivity studies. Densitometric scanning of SDS-PAGE separated extracellular proteins from hEGF recombinant...
Paper Details
Title
Bioactivity studies of Huh-7 cells derived human epidermal growth factor expressed in Pichia pastoris
Published Date
Mar 9, 2017
Volume
81
Issue
6
Pages
1114 - 1119
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