Structural, kinetic and operational characterization of an immobilized l -aminoacid dehydrogenase

l-Alanine dehydrogenase from Bacillus subtillis is a NADH-dependent enzyme that catalyzes the reversible reductive amination of pyruvate using ammonia as amine source. This enzyme has been intensively exploited in biocatalysis but its immobilized form is rarely used. In this work, we have immobilized this enzyme on agarose microbeads activated with glyoxyl groups (aliphatic aldehydes). 85% of the offered enzyme was immobilized on these...