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Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe

Published on Apr 14, 2017in Journal of Biological Chemistry
路 DOI :10.1074/jbc.M116.767806
Ling Guan1
Estimated H-index: 1
(THU: Tsinghua University),
Peng He1
Estimated H-index: 1
(THU: Tsinghua University)
+ 10 AuthorsDaochun Kong13
Estimated H-index: 13
(THU: Tsinghua University)
Abstract
  • References (46)
  • Citations (3)
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References46
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#1Young-Sang JungH-Index: 12
#2Mengli CaiH-Index: 19
Last. G. Marius CloreH-Index: 48
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Abstract The solution structure of the IIA-IIB complex of the N,N鈥-diacetylchitobiose (Chb) transporter of the Escherichia coli phosphotransferase system has been solved by NMR. The active site His-89 of IIAChb was mutated to Glu to mimic the phosphorylated state and the active site Cys-10 of IIBChb was substituted by serine to prevent intermolecular disulfide bond formation. Binding is weak with a KD of 鈭1.3 mm. The two complementary interaction surfaces are largely hydrophobic, with the protru...
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#1Remo Rohs (Columbia University)H-Index: 35
#2Xiangshu Jin (Columbia University)H-Index: 21
Last. Richard S. Mann (Columbia University)H-Index: 54
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Specific interactions between proteins and DNA are fundamental to many biological processes. In this review, we provide a revised view of protein-DNA interactions that emphasizes the importance of the threedimensional structures of both macromolecules. We divide proteinDNA interactions into two categories: those when the protein recognizes the unique chemical signatures of the DNA bases (base readout) and those when the protein recognizes a sequence-dependent DNA shape (shape readout). We furthe...
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#1Paul Emsley (University of Oxford)H-Index: 21
#2Bernhard Lohkamp (KI: Karolinska Institutet)H-Index: 11
Last. Kevin Cowtan (Ebor: University of York)H-Index: 28
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Coot is a molecular-graphics application for model building and validation of biological macromolecules. The program displays electron-density maps and atomic models and allows model manipulations such as idealization, real-space refinement, manual rotation/translation, rigid-body fitting, ligand search, solvation, mutations, rotamers and Ramachandran idealization. Furthermore, tools are provided for model validation as well as interfaces to external programs for refinement, validation and graph...
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The pre-replicative complex (pre-RC) is formed at all potential origins of replication through the action of the origin recognition complex (ORC), Cdc6, Cdt1, and the Mcm2-7 complex. The end result of pre-RC formation is the loading of the Mcm2-7 replicative helicase onto origin DNA. We examined pre-RC formation in vitro and found that it proceeds through separable binding events. Origin-bound ORC recruits Cdc6, and this ternary complex then promotes helicase loading in the presence of a pre-for...
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#1Paul D. Adams (University of California, Berkeley)H-Index: 84
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Macromolecular X-ray crystallography is routinely applied to understand biological processes at a molecular level. How颅ever, significant time and effort are still required to solve and complete many of these structures because of the need for manual interpretation of complex numerical data using many software packages and the repeated use of interactive three-dimensional graphics. PHENIX has been developed to provide a comprehensive system for macromolecular crystallo颅graphic structure solution ...
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During pre-replication complex (pre-RC) formation, origin recognition complex (ORC), Cdc6, and Cdt1 cooperatively load the 6-subunit mini chromosome maintenance (MCM2-7) complex onto DNA. Loading of MCM2-7 is a prerequisite for DNA licensing that restricts DNA replication to once per cell cycle. During S phase MCM2-7 functions as part of the replicative helicase but within the pre-RC MCM2-7 is inactive. The organization of replicative DNA helicases before and after loading onto DNA has been stud...
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#1Dirk Remus (London Research Institute)H-Index: 12
#2Fabienne Beuron (ICR: Institute of Cancer Research)H-Index: 10
Last. John F. X. Diffley (London Research Institute)H-Index: 64
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Summary The licensing of eukaryotic DNA replication origins, which ensures once-per-cell-cycle replication, involves the loading of six related minichromosome maintenance proteins (Mcm2鈥7) into prereplicative complexes (pre-RCs). Mcm2鈥7 forms the core of the replicative DNA helicase, which is inactive in the pre-RC. The loading of Mcm2鈥7 onto DNA requires the origin recognition complex (ORC), Cdc6, and Cdt1, and depends on ATP. We have reconstituted Mcm2鈥7 loading with purified budding yeast pro...
412 CitationsSource
#1Marc van Dijk (UU: Utrecht University)H-Index: 17
#2Alexandre M. J. J. Bonvin (UU: Utrecht University)H-Index: 60
There is a growing interest in structural studies of DNA by both experimental and computational approaches. Often, 3D-structural models of DNA are required, for instance, to serve as templates for homology modeling, as starting structures for macro-molecular docking or as scaffold for NMR structure calculations. The conformational adaptability of DNA when binding to a protein is often an important factor and at the same time a limitation in such studies. As a response to the demand for 3D-struct...
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#1Makoto T. Hayashi (Osaka University)H-Index: 9
#2Yuki KatouH-Index: 34
Last. Hisao Masukata (Osaka University)H-Index: 25
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DNA replication of eukaryotic chromosomes initiates at a number of discrete loci, called replication origins. Distribution and regulation of origins are important for complete duplication of the genome. Here, we determined locations of Orc1 and Mcm6, components of pre-replicative complex (pre-RC), on the whole genome of Schizosaccharomyces pombe using a high-resolution tiling array. Pre-RC sites were identified in 460 intergenic regions, where Orc1 and Mcm6 colocalized. By mapping of 5-bromo-2鈥-...
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#1Christian Heichinger (Rockefeller University)H-Index: 7
#2Christopher J. Penkett (Wellcome Trust Sanger Institute)H-Index: 11
Last. Paul Nurse (Rockefeller University)H-Index: 89
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Eukaryotic DNA replication is initiated from multiple origins of replication, but little is known about the global regulation of origins throughout the genome or in different types of cell cycles. Here, we identify 401 strong origins and 503 putative weaker origins spaced in total every 14 kb throughout the genome of the fission yeast Schizosaccharomyces pombe. The same origins are used during premeiotic and mitotic S-phases. We found that few origins fire late in mitotic S-phase and that activa...
172 CitationsSource
Cited By3
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#1J煤lia Torn茅 (University of Paris)
#2Dominique Ray-Gallet (University of Paris)H-Index: 1
Last. Genevi猫ve Almouzni (University of Paris)H-Index: 68
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The packaging of DNA into nucleosomes represents a challenge for transcription. Nucleosome disruption and histone eviction enables RNA Polymerase II progression through DNA, a process that compromises chromatin integrity and the maintenance of epigenetic information. Here, we used the imaging SNAP-tag system to distinguish new and old histones and monitor chromatin re-assembly coupled to transcription in cells. First, we uncovered a loss of both old variants H3.1 and H3.3 that depends on transcr...
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#1Lihong Wu (PKU: Peking University)
#2Yu Hua (PKU: Peking University)
Last. Daochun Kong (PKU: Peking University)H-Index: 13
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A central event in the initiation of DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) on specific chromatin sites known as DNA replication origins. The pre-RC assembly process differs between budding and fission yeasts. In fission yeast, Sap1 directly participates in pre-RC assembly, together with the four initiation factors: ORC, Cdc18/Cdc6, Cdt1, and MCM. In metazoans, the nature of DNA replication origins is not defined and the mechanism of pre-RC assembly rem...
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#1Shanna J. Smith (Beckman Research Institute)H-Index: 3
#2Caroline M. Li (Beckman Research Institute)H-Index: 4
Last. Linda H. Malkas (Beckman Research Institute)H-Index: 26
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#1Maria Louise M酶nster J酶rgensen (UCPH: University of Copenhagen)H-Index: 2
#2Babatunde Ekundayo (University of Geneva)H-Index: 2
Last. Thomas Schalch (University of Geneva)H-Index: 10
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The mechanism by which specific protein-DNA complexes induce programmed replication fork stalling in the eukaryotic genome remains poorly understood. In order to shed light on this process we carried out structural investigations on the essential fission yeast protein Sap1. Sap1 was identified as a protein involved in mating-type switching in Schizosaccharomyces pombe, and has been shown to be involved in programmed replication fork stalling. Interestingly, Sap1 assumes two different DNA binding...
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